Determination of protein concentrations and their molecular weight in tears from cats with normal corneas and cats with corneal sequestrum
Harriet J. Davidson
MS, DVM
Harriet J. Davidson
From the Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201 (Davidson) and the Department of Medicine, College of Human Medicine, Michigan State University, East Lansing, MI 48824 (Gerlach, Bull). Dr. Davidson's present address is the Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506.
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John A. Gerlach
PhD
John A. Gerlach
From the Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201 (Davidson) and the Department of Medicine, College of Human Medicine, Michigan State University, East Lansing, MI 48824 (Gerlach, Bull). Dr. Davidson's present address is the Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506.
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Robert W. Bull
DVM
Robert W. Bull
From the Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201 (Davidson) and the Department of Medicine, College of Human Medicine, Michigan State University, East Lansing, MI 48824 (Gerlach, Bull). Dr. Davidson's present address is the Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506.
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SUMMARY
Protein concentration was determined, using the Bradford technique, in tears from cats with normal corneas and from cats with corneal sequestrum. Tears from the former group contained 5.81 ± 2.29 mg of protein/ml; those from corneal sequestrum-affected cats contained 6.21 ± 2.21 mg/ml. Difference between the 2 values was not significant. Molecular weight determination was made, using 4 to 20% sodium dodecyl sulfate-polyacrylamide gels. Molecular mass of proteins ranged from 263 to 14 kDa. There was no detectable difference in the band patterns for the 2 groups.
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