Single-step technique for staining Anaplasma marginale in bovine blood smears

L. T. Hart From the Department of Veterinary Science (Hart, Morris, Todd, Enright), Experiment Statistics (Bessin), and Entomology (LePrince), Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, and the Departments of Veterinary Microbiology and Parasitology (Todd) and Veterinary Science (Luther), Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803.

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N. G. Morris From the Department of Veterinary Science (Hart, Morris, Todd, Enright), Experiment Statistics (Bessin), and Entomology (LePrince), Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, and the Departments of Veterinary Microbiology and Parasitology (Todd) and Veterinary Science (Luther), Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803.

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R. Bessin From the Department of Veterinary Science (Hart, Morris, Todd, Enright), Experiment Statistics (Bessin), and Entomology (LePrince), Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, and the Departments of Veterinary Microbiology and Parasitology (Todd) and Veterinary Science (Luther), Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803.

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D. J. LePrince From the Department of Veterinary Science (Hart, Morris, Todd, Enright), Experiment Statistics (Bessin), and Entomology (LePrince), Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, and the Departments of Veterinary Microbiology and Parasitology (Todd) and Veterinary Science (Luther), Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803.

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W. J. Todd From the Department of Veterinary Science (Hart, Morris, Todd, Enright), Experiment Statistics (Bessin), and Entomology (LePrince), Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, and the Departments of Veterinary Microbiology and Parasitology (Todd) and Veterinary Science (Luther), Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803.

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F. M. Enright From the Department of Veterinary Science (Hart, Morris, Todd, Enright), Experiment Statistics (Bessin), and Entomology (LePrince), Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, and the Departments of Veterinary Microbiology and Parasitology (Todd) and Veterinary Science (Luther), Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803.

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D. G. Luther From the Department of Veterinary Science (Hart, Morris, Todd, Enright), Experiment Statistics (Bessin), and Entomology (LePrince), Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, and the Departments of Veterinary Microbiology and Parasitology (Todd) and Veterinary Science (Luther), Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803.

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DOI:
https://doi.org/10.2460/ajvr.1992.53.10.1732
Volume/Issue:
Volume 53: Issue 10
Received:
11 Nov 1991
|
Online Publication Date:
01 Oct 1992
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SUMMARY

Three available differential stains, Camco-Quik, Diff-Quik, and Wright-Giesma were compared for detection of intraerythrocytic Anaplasma marginale in bovine blood smears. In samples where < 1% to more than 51% of the rbc were infected, statistical analysis of the data indicated no significant difference in the detection of A marginale with Camco-Quik or Diff-Quik stains. However, a significantly lower percentage of infected rbc were detected when blood smears were stained with the Wright-Giemsa stain, compared with the other 2 methods.

SUMMARY

Three available differential stains, Camco-Quik, Diff-Quik, and Wright-Giesma were compared for detection of intraerythrocytic Anaplasma marginale in bovine blood smears. In samples where < 1% to more than 51% of the rbc were infected, statistical analysis of the data indicated no significant difference in the detection of A marginale with Camco-Quik or Diff-Quik stains. However, a significantly lower percentage of infected rbc were detected when blood smears were stained with the Wright-Giemsa stain, compared with the other 2 methods.

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Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Oct 1992
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