Resistance of Pasteurella multocida A:3,4 to phagocytosis by turkey macrophages and heterophils

Barry G. Harmon From the Departments of Veterinary Pathology (Harmon, Latimer, Nunnally), Avian Medicine (Glisson), and Anatomy and Radiology (Steffens), College of Veterinary Medicine, University of Georgia, Athens, GA 30602.

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John R. Glisson From the Departments of Veterinary Pathology (Harmon, Latimer, Nunnally), Avian Medicine (Glisson), and Anatomy and Radiology (Steffens), College of Veterinary Medicine, University of Georgia, Athens, GA 30602.

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Kenneth S. Latimer From the Departments of Veterinary Pathology (Harmon, Latimer, Nunnally), Avian Medicine (Glisson), and Anatomy and Radiology (Steffens), College of Veterinary Medicine, University of Georgia, Athens, GA 30602.

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W. L. Steffens From the Departments of Veterinary Pathology (Harmon, Latimer, Nunnally), Avian Medicine (Glisson), and Anatomy and Radiology (Steffens), College of Veterinary Medicine, University of Georgia, Athens, GA 30602.

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Jean C. Nunnally From the Departments of Veterinary Pathology (Harmon, Latimer, Nunnally), Avian Medicine (Glisson), and Anatomy and Radiology (Steffens), College of Veterinary Medicine, University of Georgia, Athens, GA 30602.

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Summary

A virulent field isolate and 2 vaccine strains of Pasteurella multocida A:3,4 were compared for resistance to phagocytosis by turkey macrophages and heterophils, using in vitro assays. The least virulent vaccine strain (M-9) was phagocytosed to a greater degree than was the field isolate or the other vaccine strain (Clemson University). All 3 bacteria differed significantly from each other in the amount of capsular material present as measured by polycationic ferritin labeling and electron microscopy. Removal of the capsule with hyaluronidase resulted in a significant increase in phagocytosis of the field isolate.

Summary

A virulent field isolate and 2 vaccine strains of Pasteurella multocida A:3,4 were compared for resistance to phagocytosis by turkey macrophages and heterophils, using in vitro assays. The least virulent vaccine strain (M-9) was phagocytosed to a greater degree than was the field isolate or the other vaccine strain (Clemson University). All 3 bacteria differed significantly from each other in the amount of capsular material present as measured by polycationic ferritin labeling and electron microscopy. Removal of the capsule with hyaluronidase resulted in a significant increase in phagocytosis of the field isolate.

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