Comparison of antibody response by use of synthetic adjuvant system and Freund complete adjuvant in rabbits

Francine M. Mallon From the Departments of Laboratory Animal Science (Mallon, Landi, Hughes) and Drug Metabolism (Graichen, Conway), SmithKline & French Laboratories, King of Prussia, PA 19406.

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M. Elizabeth Graichen From the Departments of Laboratory Animal Science (Mallon, Landi, Hughes) and Drug Metabolism (Graichen, Conway), SmithKline & French Laboratories, King of Prussia, PA 19406.

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Bruce R. Conway From the Departments of Laboratory Animal Science (Mallon, Landi, Hughes) and Drug Metabolism (Graichen, Conway), SmithKline & French Laboratories, King of Prussia, PA 19406.

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Margaret S. Landi From the Departments of Laboratory Animal Science (Mallon, Landi, Hughes) and Drug Metabolism (Graichen, Conway), SmithKline & French Laboratories, King of Prussia, PA 19406.

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Howard C. Hughes From the Departments of Laboratory Animal Science (Mallon, Landi, Hughes) and Drug Metabolism (Graichen, Conway), SmithKline & French Laboratories, King of Prussia, PA 19406.

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Summary

Two commercially available synthetic adjuvant systems, trehalose dimycolate (tdm) and tdm + monophosphoryl lipid A (mpl), were compared with Freund complete adjuvant (fca) for the ability to stimulate antibody production in New Zealand White rabbits (Oryctolagus cuniculus). In addition, each animal was evaluated for adverse reactions. The antigen, rat liver microsomal epoxide hydrolase, was administered sc emulsified with fca, tdm, or tdm + mpl. Serum antibody titers were stimulated with all 3 adjuvant-antigen combinations. The highest titer was produced by use of fca; tdm + mpl produced an intermediate response, and tdm produced the lowest titer. All of the rabbits immunized with fca developed sterile subcutaneous abscesses. Rabbits immunized with tdm or tdm + mpl developed no abscesses, and only slight reactions at the injection sites. The synthetic adjuvant system tdm + mpl is recommended for use in rabbits, considering its adequate stimulation of antibody production with minimal adverse reactions.

Summary

Two commercially available synthetic adjuvant systems, trehalose dimycolate (tdm) and tdm + monophosphoryl lipid A (mpl), were compared with Freund complete adjuvant (fca) for the ability to stimulate antibody production in New Zealand White rabbits (Oryctolagus cuniculus). In addition, each animal was evaluated for adverse reactions. The antigen, rat liver microsomal epoxide hydrolase, was administered sc emulsified with fca, tdm, or tdm + mpl. Serum antibody titers were stimulated with all 3 adjuvant-antigen combinations. The highest titer was produced by use of fca; tdm + mpl produced an intermediate response, and tdm produced the lowest titer. All of the rabbits immunized with fca developed sterile subcutaneous abscesses. Rabbits immunized with tdm or tdm + mpl developed no abscesses, and only slight reactions at the injection sites. The synthetic adjuvant system tdm + mpl is recommended for use in rabbits, considering its adequate stimulation of antibody production with minimal adverse reactions.

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