Detection of chemotactic factors in preovulatory follicular fluid from mares

Elaine D. Watson From the Section of Reproductive Studies, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, PA 19348.

Search for other papers by Elaine D. Watson in
Current site
Google Scholar
PubMed
Close
 BVMS, MVM, PhD
,
Patricia L. Sertich From the Section of Reproductive Studies, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, PA 19348.

Search for other papers by Patricia L. Sertich in
Current site
Google Scholar
PubMed
Close
 MS, VMD
, and
Heidi G. Zanecosky From the Section of Reproductive Studies, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, PA 19348.

Search for other papers by Heidi G. Zanecosky in
Current site
Google Scholar
PubMed
Close
 BS

Click on author name to view affiliation information

Summary

Ovulation has been likened to an inflammatory process. Inflammatory cells accumulate in the ovulating follicle, presumably because of chemotactic factors. Chemotactic activity was measured in fluid aspirated from follicles of estrous mares 0, 12, 24, and 36 hours after ultrasonographic detection of a 35-mm follicle and iv treatment with 2,500 IU of human chorionic gonadotropin. Chemotaxis was assessed by measuring directional migration of equine neutrophils under agarose. Follicular fluid acted as a chemoattractant for neutrophils, but there was no significant difference in chemotactic activity among different time intervals after administration of human chorionic gonadotropin. On the basis of results of various treatments, chemotactic properties of serum and follicular fluid were similar. Chemotactic activity was significantly reduced by heating (56 C for 30 minutes) and by trypsinization and was virtually removed by charcoal treatment. Dialyzing the follicular fluid (3,500 and 8,000 molecular weight cut-off) significantly reduced the chemotactic activity of follicular fluid and serum. The importance of chemotactic factors in the process of ovulation in the mare is yet to be established.

Summary

Ovulation has been likened to an inflammatory process. Inflammatory cells accumulate in the ovulating follicle, presumably because of chemotactic factors. Chemotactic activity was measured in fluid aspirated from follicles of estrous mares 0, 12, 24, and 36 hours after ultrasonographic detection of a 35-mm follicle and iv treatment with 2,500 IU of human chorionic gonadotropin. Chemotaxis was assessed by measuring directional migration of equine neutrophils under agarose. Follicular fluid acted as a chemoattractant for neutrophils, but there was no significant difference in chemotactic activity among different time intervals after administration of human chorionic gonadotropin. On the basis of results of various treatments, chemotactic properties of serum and follicular fluid were similar. Chemotactic activity was significantly reduced by heating (56 C for 30 minutes) and by trypsinization and was virtually removed by charcoal treatment. Dialyzing the follicular fluid (3,500 and 8,000 molecular weight cut-off) significantly reduced the chemotactic activity of follicular fluid and serum. The importance of chemotactic factors in the process of ovulation in the mare is yet to be established.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 29 29 3
PDF Downloads 19 19 5
Advertisement