Location of open reading frames coding for equine herpesvirus type-1 glycoproteins with homology to gE and gI of herpes simplex virus

Debra M. Elton From the Department of Microbiology, University of Leeds, Leeds, LS2 9JT, UK.

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William A. Bonass From the Department of Microbiology, University of Leeds, Leeds, LS2 9JT, UK.

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Richard A. Killington From the Department of Microbiology, University of Leeds, Leeds, LS2 9JT, UK.

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David M. Meredith From the Department of Microbiology, University of Leeds, Leeds, LS2 9JT, UK.

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Ian W. Halliburton From the Department of Microbiology, University of Leeds, Leeds, LS2 9JT, UK.

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Summary

The dna fragments representing the entire short unique region and part of the repeat sequences of the equine herpesvirus type-1 genome were cloned into plasmid vectors. The approximate positions of the junctions between the short unique region and the inverted repeats were then located by restriction endonuclease mapping. Two open reading frames coding for potential glycoproteins have been identified within the short unique region, using dna sequence analysis. The predicted amino acid sequences of these open reading frames had extensive homology to the herpes simplex virus glycoproteins gE and gl and the related glycoproteins of pseudorabies virus and varicella-zoster virus.

Summary

The dna fragments representing the entire short unique region and part of the repeat sequences of the equine herpesvirus type-1 genome were cloned into plasmid vectors. The approximate positions of the junctions between the short unique region and the inverted repeats were then located by restriction endonuclease mapping. Two open reading frames coding for potential glycoproteins have been identified within the short unique region, using dna sequence analysis. The predicted amino acid sequences of these open reading frames had extensive homology to the herpes simplex virus glycoproteins gE and gl and the related glycoproteins of pseudorabies virus and varicella-zoster virus.

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