Editorial Type:
Microbiology

Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting

Clayton L. Kelling From the Departments of Veterinary Science (Kelling, Kennedy, Rump, Stine) and of Plant Pathology (Partridge) Institute of Agriculture and Natural Resources, University of Nebraska, Lincoln, NE 68583-0905, and Veterinary Medical Research Institute (Kelling, Paul), College of Veterinary Medicine, Iowa State University, Ames, IA 50011.

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 DVM, PhD
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Jane E. Kennedy From the Departments of Veterinary Science (Kelling, Kennedy, Rump, Stine) and of Plant Pathology (Partridge) Institute of Agriculture and Natural Resources, University of Nebraska, Lincoln, NE 68583-0905, and Veterinary Medical Research Institute (Kelling, Paul), College of Veterinary Medicine, Iowa State University, Ames, IA 50011.

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Kristine K. Rump From the Departments of Veterinary Science (Kelling, Kennedy, Rump, Stine) and of Plant Pathology (Partridge) Institute of Agriculture and Natural Resources, University of Nebraska, Lincoln, NE 68583-0905, and Veterinary Medical Research Institute (Kelling, Paul), College of Veterinary Medicine, Iowa State University, Ames, IA 50011.

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Lisa C. Stine From the Departments of Veterinary Science (Kelling, Kennedy, Rump, Stine) and of Plant Pathology (Partridge) Institute of Agriculture and Natural Resources, University of Nebraska, Lincoln, NE 68583-0905, and Veterinary Medical Research Institute (Kelling, Paul), College of Veterinary Medicine, Iowa State University, Ames, IA 50011.

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Prem S. Paul From the Departments of Veterinary Science (Kelling, Kennedy, Rump, Stine) and of Plant Pathology (Partridge) Institute of Agriculture and Natural Resources, University of Nebraska, Lincoln, NE 68583-0905, and Veterinary Medical Research Institute (Kelling, Paul), College of Veterinary Medicine, Iowa State University, Ames, IA 50011.

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James E. Partridge From the Departments of Veterinary Science (Kelling, Kennedy, Rump, Stine) and of Plant Pathology (Partridge) Institute of Agriculture and Natural Resources, University of Nebraska, Lincoln, NE 68583-0905, and Veterinary Medical Research Institute (Kelling, Paul), College of Veterinary Medicine, Iowa State University, Ames, IA 50011.

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DOI:
https://doi.org/10.2460/ajvr.1991.52.08.1237
Volume/Issue:
Volume 52: Issue 8
Received:
19 May 1989
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Online Publication Date:
01 Aug 1991
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Summary

Viral rna oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (bvdv) grown in medium supplemented with serum contaminated with noncytopathic bvdv from the same 3 viruses grown in cell culture free of bvdv. Oligonucleotide fingerprinting also effectively discriminated between reference Singer bvdv, nadl bvdv, and New York-1 bvdv grown in bvdv-free noncontaminated or bvdv-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral rna maybe used to determine the purity of virus stocks, as well as that of bvdv vaccines.

Summary

Viral rna oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (bvdv) grown in medium supplemented with serum contaminated with noncytopathic bvdv from the same 3 viruses grown in cell culture free of bvdv. Oligonucleotide fingerprinting also effectively discriminated between reference Singer bvdv, nadl bvdv, and New York-1 bvdv grown in bvdv-free noncontaminated or bvdv-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral rna maybe used to determine the purity of virus stocks, as well as that of bvdv vaccines.

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Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Aug 1991
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