Serologic enzyme-linked immunosorbent assay responses of calves vaccinated with a killed Mycobacterium paratuberculosis vaccine

E. Spangler From the Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.

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Lawrence E. Heider From the Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.

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Steen Beck-Nielsen From the Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.

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C. Richard Dorn From the Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.

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Summary

The purpose of this study was to document the effect of calfhood vaccination for Mycobacterium paratuberculosis on a serologic elisa. Fifteen calves vaccinated with a killed paratuherculosis vaccine and 5 unvaccinated control calves were tested from the first through the fifteenth month of life. Age of vaccination ranged from 5 to 40 days. Blood samples were collected prior to vaccination and periodically thereafter. Serum antibody was analyzed by use of the elisa. All calves were elisa-negative prior to vaccination. Thirteen of 15 vaccinated calves became elisapositive between 2 and 6 months after vaccination. The unvaccinated cohort remained elisa-negative. Widespread use of vaccine may interfere with diagnosis of paratuberculosis and with control programs that are based 0n serologic tests that measure humoral antibody.

Summary

The purpose of this study was to document the effect of calfhood vaccination for Mycobacterium paratuberculosis on a serologic elisa. Fifteen calves vaccinated with a killed paratuherculosis vaccine and 5 unvaccinated control calves were tested from the first through the fifteenth month of life. Age of vaccination ranged from 5 to 40 days. Blood samples were collected prior to vaccination and periodically thereafter. Serum antibody was analyzed by use of the elisa. All calves were elisa-negative prior to vaccination. Thirteen of 15 vaccinated calves became elisapositive between 2 and 6 months after vaccination. The unvaccinated cohort remained elisa-negative. Widespread use of vaccine may interfere with diagnosis of paratuberculosis and with control programs that are based 0n serologic tests that measure humoral antibody.

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