Characterization of eugonic fermenters group EF-4 by polyacrylamide gel electrophoresis and protein immunoblot analysis

Tracy L. Hanner From the Department of Laboratory Animal Science, North Carolina Agricultural and Technical State University, Greensboro, NC 27411.

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John W. Allen From the Department of Laboratory Animal Science, North Carolina Agricultural and Technical State University, Greensboro, NC 27411.

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Andrea Robertson-Byers From the Department of Laboratory Animal Science, North Carolina Agricultural and Technical State University, Greensboro, NC 27411.

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Steven L. Hurley From the Department of Laboratory Animal Science, North Carolina Agricultural and Technical State University, Greensboro, NC 27411.

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Summary

Whole-cell lysates and proteinase K-extracted lipopolysaccharide (lps) of 19 strains of the group eugonic fermenter- 4 (ef-4) were analyzed by electrophoresis and protein immunoblotting. These strains were isolated from dog- and cat-bite abscesses in human beings, ferret and human gastric lesions, and cat-lung infections. These strains represent 2 biovar groupings; ef-4a biovars ferment glucose and possess arginine dihydrolase activity, whereas ef-4b biovars do not. Electrophoresis of wholecell lysates could distinguish between these biovars groups. Electrophoresis of lps extracts revealed that all strains of ef-4 possess smooth chemotypes. Two strains of ef-4a reacted weakly in protein immunoblots and revealed distinct lps profiles. These studies suggests that subgroups of ef-4 biovars may exist.

Summary

Whole-cell lysates and proteinase K-extracted lipopolysaccharide (lps) of 19 strains of the group eugonic fermenter- 4 (ef-4) were analyzed by electrophoresis and protein immunoblotting. These strains were isolated from dog- and cat-bite abscesses in human beings, ferret and human gastric lesions, and cat-lung infections. These strains represent 2 biovar groupings; ef-4a biovars ferment glucose and possess arginine dihydrolase activity, whereas ef-4b biovars do not. Electrophoresis of wholecell lysates could distinguish between these biovars groups. Electrophoresis of lps extracts revealed that all strains of ef-4 possess smooth chemotypes. Two strains of ef-4a reacted weakly in protein immunoblots and revealed distinct lps profiles. These studies suggests that subgroups of ef-4 biovars may exist.

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