Isolation of a major form of pepsinogen from gastric mucosa of horses

G. Khittoo From the Department of Anatomy and Physiology, Faculty of Veterinary Medicine, University of Montreal, CP 5000, Saint-Hyacinthe, Quebec, Canada, J2S 7C6.

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L. Vermette From the Department of Anatomy and Physiology, Faculty of Veterinary Medicine, University of Montreal, CP 5000, Saint-Hyacinthe, Quebec, Canada, J2S 7C6.

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G. Nappert From the Department of Anatomy and Physiology, Faculty of Veterinary Medicine, University of Montreal, CP 5000, Saint-Hyacinthe, Quebec, Canada, J2S 7C6.

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N. Lariviere From the Department of Anatomy and Physiology, Faculty of Veterinary Medicine, University of Montreal, CP 5000, Saint-Hyacinthe, Quebec, Canada, J2S 7C6.

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SUMMARY

In mammalian species studied previously, pepsinogen consisted of biochemically different groups of isozymogens. By use of gel filtration chromatography and electrophoresis, we isolated a predominant pepsinogen from the gastric mucosa of a horse. Peptide mapping with V8 protease revealed differences with its porcine homologue. However, porcine and equine pepsinogens, when activated to pepsin, had a similar pattern of activity when hemoglobin was used as substrate. Those results suggest that differences must exist in the primary structure of the pepsinogens of the 2 species.

SUMMARY

In mammalian species studied previously, pepsinogen consisted of biochemically different groups of isozymogens. By use of gel filtration chromatography and electrophoresis, we isolated a predominant pepsinogen from the gastric mucosa of a horse. Peptide mapping with V8 protease revealed differences with its porcine homologue. However, porcine and equine pepsinogens, when activated to pepsin, had a similar pattern of activity when hemoglobin was used as substrate. Those results suggest that differences must exist in the primary structure of the pepsinogens of the 2 species.

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