Immunocytochemical study of tissues from clinically normal dogs and of neoplasms, using keratin monoclonal antibodies

G. E. Sandusky From the Toxicology Division, Lilly Research Laboratories, Division of Eli Lilly & Co, Greenfield, IN 46140 (Sandusky, Wightman) and the Department of Veterinary Microbiology & Pathology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907 (Carlton).

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K. A. Wightman From the Toxicology Division, Lilly Research Laboratories, Division of Eli Lilly & Co, Greenfield, IN 46140 (Sandusky, Wightman) and the Department of Veterinary Microbiology & Pathology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907 (Carlton).

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W.W. Carlton From the Toxicology Division, Lilly Research Laboratories, Division of Eli Lilly & Co, Greenfield, IN 46140 (Sandusky, Wightman) and the Department of Veterinary Microbiology & Pathology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907 (Carlton).

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SUMMARY

Three commonly used keratin monoclonal antibodies (mab)-AE1:AE3, CAM 5.2, and MAK-6—were compared with routinely used cytokeratin antibody. The expression of these antibodies was analyzed in several tissues obtained from clinically normal dogs and in a variety of neoplasms from dogs. Using appropriate enzymatic digestion, paraffin-embedded tissues processed in routine manner retained their typical keratin expression. Differentiated and poorly differentiated epithelial neoplasms, lymphomas, and melanomas were studied by use of the avidin- biotin-peroxidase technique. All 4 of the aforementioned antibodies had similar staining profiles. Of 3 anaplastic carcinomas, 2 had positive reaction to all 4 antibodies. All lymphomas, plasma cell tumors, and amelanotic melanomas had negative reaction to MAK-6, CAM 5.2, AE1:AE3, and cytokeratin mab. Three basal cell epitheliomas had positive reaction to all 4 antibodies, whereas 1 basal cell tumor with a solid pattern had negative staining reaction. Two carcinoids had negative reaction to all markers and 1 of 2 malignant chemodectomas and 1 transitional cell carcinoma had staining reaction to only AE1:AE3 mab. Comparing the 4 antibodies, use of AE1:AE3 mab produced the strongest staining intensity followed by cytokeratin, MAK-6, and CAM 5.2 mab. All 4 antibodies had low background staining. In conclusion, AE1:AE3 and MAK-6 mab are as useful as cytokeratin mab for identification of poorly differentiated epithelial neoplasms in dogs and cats.

SUMMARY

Three commonly used keratin monoclonal antibodies (mab)-AE1:AE3, CAM 5.2, and MAK-6—were compared with routinely used cytokeratin antibody. The expression of these antibodies was analyzed in several tissues obtained from clinically normal dogs and in a variety of neoplasms from dogs. Using appropriate enzymatic digestion, paraffin-embedded tissues processed in routine manner retained their typical keratin expression. Differentiated and poorly differentiated epithelial neoplasms, lymphomas, and melanomas were studied by use of the avidin- biotin-peroxidase technique. All 4 of the aforementioned antibodies had similar staining profiles. Of 3 anaplastic carcinomas, 2 had positive reaction to all 4 antibodies. All lymphomas, plasma cell tumors, and amelanotic melanomas had negative reaction to MAK-6, CAM 5.2, AE1:AE3, and cytokeratin mab. Three basal cell epitheliomas had positive reaction to all 4 antibodies, whereas 1 basal cell tumor with a solid pattern had negative staining reaction. Two carcinoids had negative reaction to all markers and 1 of 2 malignant chemodectomas and 1 transitional cell carcinoma had staining reaction to only AE1:AE3 mab. Comparing the 4 antibodies, use of AE1:AE3 mab produced the strongest staining intensity followed by cytokeratin, MAK-6, and CAM 5.2 mab. All 4 antibodies had low background staining. In conclusion, AE1:AE3 and MAK-6 mab are as useful as cytokeratin mab for identification of poorly differentiated epithelial neoplasms in dogs and cats.

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