Radioimmunoassay of parathyroid hormone in cats

James A. Flanders From the Department of Clinical Sciences (Flanders), and the Diagnostic Laboratory (Reimers), Now York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.

Search for other papers by James A. Flanders in
Current site
Google Scholar
PubMed
Close
 DVM
and
Thomas J. Reimers From the Department of Clinical Sciences (Flanders), and the Diagnostic Laboratory (Reimers), Now York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.

Search for other papers by Thomas J. Reimers in
Current site
Google Scholar
PubMed
Close
 PhD

Click on author name to view affiliation information

SUMMARY

A radioimmunoassay for measurement of midmolecule parathyroid hormone (pth) concentration in serum from dogs was validated for use on serum from cats. The assay detected an increase in serum concentration of pth after iv infusion of Na2 edta in healthy cats. Infusion of calcium chloride caused a decrease in measured pth. Accuracy of the assay was demonstrated by quantitative recovery of a feline parathyroid gland extract added to pooled feline sera. Mean interassay and intra-assay coefficients of variation were 0.13 and 0.07, respectively. Sensitivity of the assay was 0.1 ng of pth/ml. The median pth concentration measured in 40 adult cats was 3.5 ng/ml, with a range of 1.16 to 11.0 ng/ml.

SUMMARY

A radioimmunoassay for measurement of midmolecule parathyroid hormone (pth) concentration in serum from dogs was validated for use on serum from cats. The assay detected an increase in serum concentration of pth after iv infusion of Na2 edta in healthy cats. Infusion of calcium chloride caused a decrease in measured pth. Accuracy of the assay was demonstrated by quantitative recovery of a feline parathyroid gland extract added to pooled feline sera. Mean interassay and intra-assay coefficients of variation were 0.13 and 0.07, respectively. Sensitivity of the assay was 0.1 ng of pth/ml. The median pth concentration measured in 40 adult cats was 3.5 ng/ml, with a range of 1.16 to 11.0 ng/ml.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 50 50 13
PDF Downloads 26 26 4
Advertisement