Characterization of encephalomyocarditis virus isolated from aborted swine fetuses

H. S. Kim From the Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108.

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W. T. Christianson From the Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108.

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H. S. Joo From the Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108.

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SUMMARY

Characteristics of 2 encephalomyocarditis virus (emcv) isolates (MN-25 and MN-30) recovered from aborted swine fetuses were examined along with 2 other swine isolates (NVSL-MDV and NVSL-PR) and a reference ATCC strain (VR-129). All 5 emcv isolates were found to be serologically related by cross testing, using serum neutralization and fluorescent antibody assays. Hemagglutination (ha) properties of the 5 isolates were compared, using 5 diluents. The MN-25 and NVSL-MDV strains had ha activity with guinea pig rbc in all 5 diluents, whereas MN-30, NVSL-PR, and VR-129 had ha activity only in KCl-borate buffer. The ha ability with rbc of various animal species was examined, using KCl-borate diluent. All virus isolates had high ha titer (1:512 to 1:2,048) with guinea pig, rat, and horse rbc and lower ha titer (1:16 to 1:64) with sheep rbc. The MN-25 and NVSL-MDV isolates agglutinated dog rbc, whereas MN-30, NVSL-PR, and VR-129 strains did not. Viral replication was evident in 8 of 10 cell lines tested, although infectivity titers of each virus varied by cell line used. Plaque-forming ability was similar for all 5 isolates, but plaque size was different by virus and cell culture used. Virus isolates were found to be stable after being heated at 56 C and subjected to a wide range of pH. A viral polypeptide pattern difference for all 5 isolates was not found by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It was concluded that MN-25 and MN-30 are serologically related and have similar viral characteristics as those of previous emcv isolates and the reference ATCC strain, although differences in ha ability could be observed.

SUMMARY

Characteristics of 2 encephalomyocarditis virus (emcv) isolates (MN-25 and MN-30) recovered from aborted swine fetuses were examined along with 2 other swine isolates (NVSL-MDV and NVSL-PR) and a reference ATCC strain (VR-129). All 5 emcv isolates were found to be serologically related by cross testing, using serum neutralization and fluorescent antibody assays. Hemagglutination (ha) properties of the 5 isolates were compared, using 5 diluents. The MN-25 and NVSL-MDV strains had ha activity with guinea pig rbc in all 5 diluents, whereas MN-30, NVSL-PR, and VR-129 had ha activity only in KCl-borate buffer. The ha ability with rbc of various animal species was examined, using KCl-borate diluent. All virus isolates had high ha titer (1:512 to 1:2,048) with guinea pig, rat, and horse rbc and lower ha titer (1:16 to 1:64) with sheep rbc. The MN-25 and NVSL-MDV isolates agglutinated dog rbc, whereas MN-30, NVSL-PR, and VR-129 strains did not. Viral replication was evident in 8 of 10 cell lines tested, although infectivity titers of each virus varied by cell line used. Plaque-forming ability was similar for all 5 isolates, but plaque size was different by virus and cell culture used. Virus isolates were found to be stable after being heated at 56 C and subjected to a wide range of pH. A viral polypeptide pattern difference for all 5 isolates was not found by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It was concluded that MN-25 and MN-30 are serologically related and have similar viral characteristics as those of previous emcv isolates and the reference ATCC strain, although differences in ha ability could be observed.

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