Effect of vaccination with a monovalent Leptospira interrogans serovar hardjo type hardjo-bovis vaccine on type hardjo-bovis infection of cattle

Carole A. Bolin From the Leptospirosis/Mycobacteriosis Research Unit, National Animal Disease Center, USDA, Agriculture Research Service, PO Box 70, Ames, IA 50010 (Bolin, Zuerner, Trueba) and the Veterinary Research Laboratories, Stormont, North Ireland (Cassells).

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James A. Cassells From the Leptospirosis/Mycobacteriosis Research Unit, National Animal Disease Center, USDA, Agriculture Research Service, PO Box 70, Ames, IA 50010 (Bolin, Zuerner, Trueba) and the Veterinary Research Laboratories, Stormont, North Ireland (Cassells).

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Richard L. Zuerner From the Leptospirosis/Mycobacteriosis Research Unit, National Animal Disease Center, USDA, Agriculture Research Service, PO Box 70, Ames, IA 50010 (Bolin, Zuerner, Trueba) and the Veterinary Research Laboratories, Stormont, North Ireland (Cassells).

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Gabriel Trueba From the Leptospirosis/Mycobacteriosis Research Unit, National Animal Disease Center, USDA, Agriculture Research Service, PO Box 70, Ames, IA 50010 (Bolin, Zuerner, Trueba) and the Veterinary Research Laboratories, Stormont, North Ireland (Cassells).

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 DVM, MS

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SUMMARY

Effectiveness of 2 concentrations of a monovalent vaccine containing Leptospira interrogans serovar hardjo type hardjo-bovis was evaluated for protection of heifers from infection with type hardjo-bovis. Nine heifers were given 2 doses of low-dose vaccine (8.32 × 108 cells/dose); 9 heifers were given 2 doses of high-dose vaccine (8.32 × 109 cells/dose); and 1 steer and 1 heifer were maintained as nonvaccinated controls. Groups of vaccinated cattle were challenge-exposed with serovar hardjo type hardjo-bovis at 7 (n = 6), 11 (n = 6), or 15 (n = 6) weeks after completion of vaccination. All cattle were challenge-exposed by conjunctival instillation of 1 × 105 hardjo-bovis cells on 3 consecutive days. Both control and all vaccinated cattle became infected and shed serovar hardjo type hardjo-bovis in their urine. Leptospires were detected in 15 of 16 (94%) urine samples from control cattle and in 124 of 143 (87%) samples from vaccinated cattle. Leptospires were detected in kidneys of 17 of 18 vaccinated cattle and 2 of 2 control cattle and in the uterus or oviducts of 13 of 18 vaccinates and the 1 control heifer.

SUMMARY

Effectiveness of 2 concentrations of a monovalent vaccine containing Leptospira interrogans serovar hardjo type hardjo-bovis was evaluated for protection of heifers from infection with type hardjo-bovis. Nine heifers were given 2 doses of low-dose vaccine (8.32 × 108 cells/dose); 9 heifers were given 2 doses of high-dose vaccine (8.32 × 109 cells/dose); and 1 steer and 1 heifer were maintained as nonvaccinated controls. Groups of vaccinated cattle were challenge-exposed with serovar hardjo type hardjo-bovis at 7 (n = 6), 11 (n = 6), or 15 (n = 6) weeks after completion of vaccination. All cattle were challenge-exposed by conjunctival instillation of 1 × 105 hardjo-bovis cells on 3 consecutive days. Both control and all vaccinated cattle became infected and shed serovar hardjo type hardjo-bovis in their urine. Leptospires were detected in 15 of 16 (94%) urine samples from control cattle and in 124 of 143 (87%) samples from vaccinated cattle. Leptospires were detected in kidneys of 17 of 18 vaccinated cattle and 2 of 2 control cattle and in the uterus or oviducts of 13 of 18 vaccinates and the 1 control heifer.

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