Development of surgical techniques for preparation of in vitro-isolated perfused porcine skin flaps for percutaneous absorption studies

K. F. Bowman From the Laboratory of Surgical Research (Bowman), Department of Food Animal and Equine Medicine; and the Cutaneous Pharmacology and Toxicology Center (Monteiro-Riviere, Riviere), College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough St, Raleigh, NC 27606.

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 DVM, MS
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Nancy A. Monteiro-Riviere From the Laboratory of Surgical Research (Bowman), Department of Food Animal and Equine Medicine; and the Cutaneous Pharmacology and Toxicology Center (Monteiro-Riviere, Riviere), College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough St, Raleigh, NC 27606.

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J. E. Riviere From the Laboratory of Surgical Research (Bowman), Department of Food Animal and Equine Medicine; and the Cutaneous Pharmacology and Toxicology Center (Monteiro-Riviere, Riviere), College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough St, Raleigh, NC 27606.

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SUMMARY

We developed a single-pedicle, axial pattern tubed skin flap that could be transferred to an in vitro perfusion apparatus. On the basis of results of prosections, angiography, contact radiography, and surviving-length studies, it was concluded that a single-pedicle, axial pattern skin flap measuring 4 cm × 12 cm incorporating the caudal superficial epigastric artery would survive to its entire length. Subsequently, a surgical (stage 1) procedure was developed for the routine preparation of single-pedicle, axial pattern tubed skin flaps. Healing after the stage-1 procedure was evaluated by visual inspection and fluorescein angiography. Stage-1 procedures were performed successfully 149 of 160 (93%) times. A second surgical (stage 2) procedure was developed for routine cannulation of the caudal superficial epigastric artery and harvest of the tubed skin flap. Stage-2 procedures were performed successfully 136 of 144 (94%) times.

SUMMARY

We developed a single-pedicle, axial pattern tubed skin flap that could be transferred to an in vitro perfusion apparatus. On the basis of results of prosections, angiography, contact radiography, and surviving-length studies, it was concluded that a single-pedicle, axial pattern skin flap measuring 4 cm × 12 cm incorporating the caudal superficial epigastric artery would survive to its entire length. Subsequently, a surgical (stage 1) procedure was developed for the routine preparation of single-pedicle, axial pattern tubed skin flaps. Healing after the stage-1 procedure was evaluated by visual inspection and fluorescein angiography. Stage-1 procedures were performed successfully 149 of 160 (93%) times. A second surgical (stage 2) procedure was developed for routine cannulation of the caudal superficial epigastric artery and harvest of the tubed skin flap. Stage-2 procedures were performed successfully 136 of 144 (94%) times.

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