Prophylactic efficacy of four antibacterial shampoos against Staphylococcus intermedius in dogs

Kenneth W. Kwochka From the Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, 1935 Coffey Rd, Columbus, OH 43210.

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Joseph J. Kowalski From the Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, 1935 Coffey Rd, Columbus, OH 43210.

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SUMMARY

Prophylactic efficacy of 4 antibacterial shampoos against Staphylococcus intermedins in dogs was determined by use of a controlled quantitative technique. Ten adult Beagles were used in the study. The antibacterial agents in the shampoos were 3.0% benzoyl peroxide, 0.5% Chlorhexidine acetate, 1.0% available iodine as a polyalkyle-neglycol-iodine complex, and a combination of 0.5% triclosan, 2.0% sulfur, and 2.0% salicylic acid. Treated and control sites were challenge exposed with 5.30 ± 0.10 (log10) S intermedins colony-forming units (cfu)/cm2 of skin and occluded for 5 hours. At the end of the test period, remaining bacteria were removed with a detergent cup-scrub technique and the total number of S intermedins cfu/cm2 skin was calculated for each treated and control site.

Nontreated bacteria-challenged control sites yielded 5.62 ± 0.65 S intermedins cfu/cm2 of skin. Staphylococcus intermedins recovery (cfu/cm2) from the treated sites was 0.94 ± 0.76 for benzoyl peroxide, 1.96 ± 1.33 for Chlorhexidine acetate, 3.11 ± 0.48 for organic iodine, and 4.69 ± 0.23 for triclosan-sulfur-salicylic acid. Each S intermedins recovery value from the 4 treated sites was significantly (P < 0.05) lower than that from the nontreated S intermedins challenge-exposed control site. Bacteria recovery values were also significantly (P < 0.05) different among the 4 shampoo-treated sites.

We concluded that all shampoos had significant (P < 0.05) prophylactic activity against S intermedins over 5 hours. The shampoo containing benzoyl peroxide was determined to have the greatest efficacy among the products tested.

SUMMARY

Prophylactic efficacy of 4 antibacterial shampoos against Staphylococcus intermedins in dogs was determined by use of a controlled quantitative technique. Ten adult Beagles were used in the study. The antibacterial agents in the shampoos were 3.0% benzoyl peroxide, 0.5% Chlorhexidine acetate, 1.0% available iodine as a polyalkyle-neglycol-iodine complex, and a combination of 0.5% triclosan, 2.0% sulfur, and 2.0% salicylic acid. Treated and control sites were challenge exposed with 5.30 ± 0.10 (log10) S intermedins colony-forming units (cfu)/cm2 of skin and occluded for 5 hours. At the end of the test period, remaining bacteria were removed with a detergent cup-scrub technique and the total number of S intermedins cfu/cm2 skin was calculated for each treated and control site.

Nontreated bacteria-challenged control sites yielded 5.62 ± 0.65 S intermedins cfu/cm2 of skin. Staphylococcus intermedins recovery (cfu/cm2) from the treated sites was 0.94 ± 0.76 for benzoyl peroxide, 1.96 ± 1.33 for Chlorhexidine acetate, 3.11 ± 0.48 for organic iodine, and 4.69 ± 0.23 for triclosan-sulfur-salicylic acid. Each S intermedins recovery value from the 4 treated sites was significantly (P < 0.05) lower than that from the nontreated S intermedins challenge-exposed control site. Bacteria recovery values were also significantly (P < 0.05) different among the 4 shampoo-treated sites.

We concluded that all shampoos had significant (P < 0.05) prophylactic activity against S intermedins over 5 hours. The shampoo containing benzoyl peroxide was determined to have the greatest efficacy among the products tested.

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