Bovine recombinant granulocyte-macrophage colony-stimulating factor enhancement of bovine neutrophil functions in vitro

P. G. Reddy From the Departments of Anatomy and Physiology (Reddy, Blecha), and Laboratory Medicine (McVey, Chengappa, Minocha), College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, and Immunex Corp, University Road, Seattle, WA 98101 (Baker).

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D. S. McVey From the Departments of Anatomy and Physiology (Reddy, Blecha), and Laboratory Medicine (McVey, Chengappa, Minocha), College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, and Immunex Corp, University Road, Seattle, WA 98101 (Baker).

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M. M. Chengappa From the Departments of Anatomy and Physiology (Reddy, Blecha), and Laboratory Medicine (McVey, Chengappa, Minocha), College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, and Immunex Corp, University Road, Seattle, WA 98101 (Baker).

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F. Blecha From the Departments of Anatomy and Physiology (Reddy, Blecha), and Laboratory Medicine (McVey, Chengappa, Minocha), College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, and Immunex Corp, University Road, Seattle, WA 98101 (Baker).

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H. C. Minocha From the Departments of Anatomy and Physiology (Reddy, Blecha), and Laboratory Medicine (McVey, Chengappa, Minocha), College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, and Immunex Corp, University Road, Seattle, WA 98101 (Baker).

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P. E. Baker From the Departments of Anatomy and Physiology (Reddy, Blecha), and Laboratory Medicine (McVey, Chengappa, Minocha), College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, and Immunex Corp, University Road, Seattle, WA 98101 (Baker).

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SUMMARY

Neutrophils were purified from blood of dexamethasone-treated (0.04 mg/kg of body weight) and untreated calves. Cells were untreated (controls) or cultured in media containing 5 or 10 ng of bovine recombinant granulocyte-macrophage colony-stimulating factor (rbgm-csf)/ml for 10 to 12 hours before being tested for various functions. Dexamethasone treatment of calves decreased luminol-dependent chemiluminescence, decreased phagocytosis of Pasteurella multocida and several Staphylococcus spp by various degrees, and decreased antibody-dependent cell-mediated cytotoxocity against bovine herpesvirus-infected cells by 26 to 32%. The percentage phagocytosis of coagulase-positive S aureus and S intermedius was higher than that of coagulase-negative S epidermidis for neutrophils from all calves. Culture of neutrophils with rbgm-csf significantly increased (P < 0.05) all of the aforementioned functions, compared with control neutrophils; however, rbgm-csf-induced increases in function tended to be higher in neutrophils from dexamethasone-treated calves than in neutrophils from untreated calves.

SUMMARY

Neutrophils were purified from blood of dexamethasone-treated (0.04 mg/kg of body weight) and untreated calves. Cells were untreated (controls) or cultured in media containing 5 or 10 ng of bovine recombinant granulocyte-macrophage colony-stimulating factor (rbgm-csf)/ml for 10 to 12 hours before being tested for various functions. Dexamethasone treatment of calves decreased luminol-dependent chemiluminescence, decreased phagocytosis of Pasteurella multocida and several Staphylococcus spp by various degrees, and decreased antibody-dependent cell-mediated cytotoxocity against bovine herpesvirus-infected cells by 26 to 32%. The percentage phagocytosis of coagulase-positive S aureus and S intermedius was higher than that of coagulase-negative S epidermidis for neutrophils from all calves. Culture of neutrophils with rbgm-csf significantly increased (P < 0.05) all of the aforementioned functions, compared with control neutrophils; however, rbgm-csf-induced increases in function tended to be higher in neutrophils from dexamethasone-treated calves than in neutrophils from untreated calves.

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