Summary
Canine circulating neutrophils, isolated by a blood lysing technique, were incubated with 7-S nerve growth factor (ngf), at final concentrations between 12.5 and 800 ng/ml, for 30 minutes at 37 C. Neutrophil cytosolic H2O2 production, measured by flow cytometry, after 7-S ngf incubation was not significantly different from that produced at 37 C (baseline temperature controls) alone. Phorbol myristate acetate (pma; 100 ng/ml) stimulation of neutrophils produced cytosolic H2O2 concentrations almost 13 times that of baseline temperature control neutrophils. Preincubation of neutrophils with 7-S ngf (100 to 800 ng/ml, 30 minutes, 37 C) and subsequent stimulation by pma resulted in augmented H2O2 production in excess of twice that of neutrophils treated with pma alone, and almost 30 times that of baseline temperature controls.
