Pharmacokinetics of single-dose intravenous or intramuscular administration of gentamicin in roosters

W. M. Pedersoli From the Departments of Physiology and Pharmacology (Pedersoli, Askins), Anatomy and Histology (Krista, Whitesides), and Pathology and Parasitology (Spano), College of Veterinary Medicine, and the Department of Pharmacal Sciences, School of Pharmacy (Ravis, Tolbert), Auburn University, AL 36849.

Search for other papers by W. M. Pedersoli in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
W. R. Ravis From the Departments of Physiology and Pharmacology (Pedersoli, Askins), Anatomy and Histology (Krista, Whitesides), and Pathology and Parasitology (Spano), College of Veterinary Medicine, and the Department of Pharmacal Sciences, School of Pharmacy (Ravis, Tolbert), Auburn University, AL 36849.

Search for other papers by W. R. Ravis in
Current site
Google Scholar
PubMed
Close
 PhD
,
D. R. Askins From the Departments of Physiology and Pharmacology (Pedersoli, Askins), Anatomy and Histology (Krista, Whitesides), and Pathology and Parasitology (Spano), College of Veterinary Medicine, and the Department of Pharmacal Sciences, School of Pharmacy (Ravis, Tolbert), Auburn University, AL 36849.

Search for other papers by D. R. Askins in
Current site
Google Scholar
PubMed
Close
 BS
,
L. M. Krista From the Departments of Physiology and Pharmacology (Pedersoli, Askins), Anatomy and Histology (Krista, Whitesides), and Pathology and Parasitology (Spano), College of Veterinary Medicine, and the Department of Pharmacal Sciences, School of Pharmacy (Ravis, Tolbert), Auburn University, AL 36849.

Search for other papers by L. M. Krista in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
J. S. Spano From the Departments of Physiology and Pharmacology (Pedersoli, Askins), Anatomy and Histology (Krista, Whitesides), and Pathology and Parasitology (Spano), College of Veterinary Medicine, and the Department of Pharmacal Sciences, School of Pharmacy (Ravis, Tolbert), Auburn University, AL 36849.

Search for other papers by J. S. Spano in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
J. F. Whitesides From the Departments of Physiology and Pharmacology (Pedersoli, Askins), Anatomy and Histology (Krista, Whitesides), and Pathology and Parasitology (Spano), College of Veterinary Medicine, and the Department of Pharmacal Sciences, School of Pharmacy (Ravis, Tolbert), Auburn University, AL 36849.

Search for other papers by J. F. Whitesides in
Current site
Google Scholar
PubMed
Close
 MS
, and
D. S. Tolbert From the Departments of Physiology and Pharmacology (Pedersoli, Askins), Anatomy and Histology (Krista, Whitesides), and Pathology and Parasitology (Spano), College of Veterinary Medicine, and the Department of Pharmacal Sciences, School of Pharmacy (Ravis, Tolbert), Auburn University, AL 36849.

Search for other papers by D. S. Tolbert in
Current site
Google Scholar
PubMed
Close
 BS

Click on author name to view affiliation information

SUMMARY

Healthy mature roosters (n = 10) were given gentamicin (5 mg/kg of body weight, IV) and, 30 days later, another dose im Serum concentrations of gentamicin were determined over 60 hours after each drug dosing, using a radioimmunoassay. Using nonlinear least-square regression methods, the combined data of IV and im treatments were best fitted by a 2-compartment open model. The mean distribution phase half-life was 0.203 ± 0.075 hours (mean ± SD) and the terminal half-life was 3.38 ± 0.62 hours. The volume of the central compartment was 0.0993 ± 0.0097 L/kg, volume of distribution at steady state was 0.209 ± 0.013 L/kg, and the total body clearance was 46.5 ± 7.9 ml/h/kg.

Intramuscular absorption was rapid, with a half-life for absorption of 0.281 ± 0.081 hours. The extent of im absorption was 95 ± 18%. Maximal serum concentration of 20.68 ± 2.10 μg/ml was detected at 0.62 ± 0.18 hours after the dose. Kinetic calculations predicted that IM injection of gentamicin at a dosage of 4 mg/kg, q 12 h, and 1.5 mg/kg, q 8 h, would provide average steady-state serum concentrations of 6.82 and 3.83 μg/ml, with minimal steady-state serum concentrations of 1.54 and 1.50 μg/ml and maximal steady-state serum concentrations of 18.34 and 7.70 μg/ml, respectively.

SUMMARY

Healthy mature roosters (n = 10) were given gentamicin (5 mg/kg of body weight, IV) and, 30 days later, another dose im Serum concentrations of gentamicin were determined over 60 hours after each drug dosing, using a radioimmunoassay. Using nonlinear least-square regression methods, the combined data of IV and im treatments were best fitted by a 2-compartment open model. The mean distribution phase half-life was 0.203 ± 0.075 hours (mean ± SD) and the terminal half-life was 3.38 ± 0.62 hours. The volume of the central compartment was 0.0993 ± 0.0097 L/kg, volume of distribution at steady state was 0.209 ± 0.013 L/kg, and the total body clearance was 46.5 ± 7.9 ml/h/kg.

Intramuscular absorption was rapid, with a half-life for absorption of 0.281 ± 0.081 hours. The extent of im absorption was 95 ± 18%. Maximal serum concentration of 20.68 ± 2.10 μg/ml was detected at 0.62 ± 0.18 hours after the dose. Kinetic calculations predicted that IM injection of gentamicin at a dosage of 4 mg/kg, q 12 h, and 1.5 mg/kg, q 8 h, would provide average steady-state serum concentrations of 6.82 and 3.83 μg/ml, with minimal steady-state serum concentrations of 1.54 and 1.50 μg/ml and maximal steady-state serum concentrations of 18.34 and 7.70 μg/ml, respectively.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 31 31 4
PDF Downloads 33 33 1
Advertisement