Outer membrane protein profiles of Ediwardsiella ictaluri from fish

Joseph C. Newton From the Department of Pathobiology, College of Veterinary Medicine (Newton, Wolfe, Wilt), and the Department of Botany and Microbiology, College of Sciences and Mathematics (Blevins), Auburn University, AL 36849-3501.

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Willard T. Blevins From the Department of Pathobiology, College of Veterinary Medicine (Newton, Wolfe, Wilt), and the Department of Botany and Microbiology, College of Sciences and Mathematics (Blevins), Auburn University, AL 36849-3501.

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Gerald R. Wilt From the Department of Pathobiology, College of Veterinary Medicine (Newton, Wolfe, Wilt), and the Department of Botany and Microbiology, College of Sciences and Mathematics (Blevins), Auburn University, AL 36849-3501.

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Lauren G. Wolfe From the Department of Pathobiology, College of Veterinary Medicine (Newton, Wolfe, Wilt), and the Department of Botany and Microbiology, College of Sciences and Mathematics (Blevins), Auburn University, AL 36849-3501.

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SUMMARY

Outer membrane proteins (omp) prepared with sodium N-lauroyl sarcocinate (sls) from 33 Edwardsiella ictaluri isolates from fish were examined by electrophoresis. Twenty-eight isolates from channel catfish (Ictalurus punctatus) had similar OMP profiles. Ten bands (71 kilodaltons [kD] to 19.5 kD) were identified in all isolates from channel catfish. One major 35-kD protein comprised most of the protein content of the outer membrane of isolates from channel catfish. Differences existed among isolates in the amount of protein within minor omp bands. Edwardsiella ictaluri ATCC 33202 contained larger quantities of the 38.5- and 37-kD proteins than did the other isolates.

SUMMARY

Outer membrane proteins (omp) prepared with sodium N-lauroyl sarcocinate (sls) from 33 Edwardsiella ictaluri isolates from fish were examined by electrophoresis. Twenty-eight isolates from channel catfish (Ictalurus punctatus) had similar OMP profiles. Ten bands (71 kilodaltons [kD] to 19.5 kD) were identified in all isolates from channel catfish. One major 35-kD protein comprised most of the protein content of the outer membrane of isolates from channel catfish. Differences existed among isolates in the amount of protein within minor omp bands. Edwardsiella ictaluri ATCC 33202 contained larger quantities of the 38.5- and 37-kD proteins than did the other isolates.

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