Effects of exocrine pancreatic insufficiency and replacement therapy on the bacterial flora of the duodenum in dogs

Kenneth W. Simpson From the Departments of Biomedical Services (Simpson, Morton) and Microbiology (Jones), University of Leicester, LE1 7RH, and the Department of Veterinary Pathology (Batt), University of Liverpool, PO Box 147, Liverpool L69 3BX, UK.

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 BVM&S, PhD
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Roger M. Batt From the Departments of Biomedical Services (Simpson, Morton) and Microbiology (Jones), University of Leicester, LE1 7RH, and the Department of Veterinary Pathology (Batt), University of Liverpool, PO Box 147, Liverpool L69 3BX, UK.

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Dorothy Jones From the Departments of Biomedical Services (Simpson, Morton) and Microbiology (Jones), University of Leicester, LE1 7RH, and the Department of Veterinary Pathology (Batt), University of Liverpool, PO Box 147, Liverpool L69 3BX, UK.

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David B. Morton From the Departments of Biomedical Services (Simpson, Morton) and Microbiology (Jones), University of Leicester, LE1 7RH, and the Department of Veterinary Pathology (Batt), University of Liverpool, PO Box 147, Liverpool L69 3BX, UK.

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 BVSc, PhD

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SUMMARY

The influence of pancreatic secretions on the bacterial flora of the small intestine in 6 dogs was investigated by determining effects of exocrine pancreatic insufficiency on numbers and types of bacteria in duodenal juice, and by examining the subsequent response to dietary supplementation with bovine pancreatic extract. Exocrine pancreatic insufficiency was induced by ligation of pancreatic ducts and was confirmed by indirect assessment of exocrine pancreatic function. Duct ligation was followed by large increases (P < 0.01) in total numbers of bacteria, reflecting increased numbers particularly of Lactobacillus spp and Streptococcus spp, in 3 dogs accompanied by obligate anaerobes. Total numbers of aerobes and anaerobes decreased markedly (P < 0.05) after supplementation with bovine pancreatic extract to values that were not significantly different from those determined before duct ligation. Exocrine pancreatic insufficiency therefore resulted in small intestinal bacterial overgrowth that was reversed by pancreatic replacement therapy, indicating that pancreatic secretions can have an important influence on the small intestinal bacterial flora of dogs.

SUMMARY

The influence of pancreatic secretions on the bacterial flora of the small intestine in 6 dogs was investigated by determining effects of exocrine pancreatic insufficiency on numbers and types of bacteria in duodenal juice, and by examining the subsequent response to dietary supplementation with bovine pancreatic extract. Exocrine pancreatic insufficiency was induced by ligation of pancreatic ducts and was confirmed by indirect assessment of exocrine pancreatic function. Duct ligation was followed by large increases (P < 0.01) in total numbers of bacteria, reflecting increased numbers particularly of Lactobacillus spp and Streptococcus spp, in 3 dogs accompanied by obligate anaerobes. Total numbers of aerobes and anaerobes decreased markedly (P < 0.05) after supplementation with bovine pancreatic extract to values that were not significantly different from those determined before duct ligation. Exocrine pancreatic insufficiency therefore resulted in small intestinal bacterial overgrowth that was reversed by pancreatic replacement therapy, indicating that pancreatic secretions can have an important influence on the small intestinal bacterial flora of dogs.

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