Effect of muramyl dipeptide on immunogenicity of Corynebacterium pseudotuberculosis whole-cell vaccines in mice and lambs

Kim A. Brogden From the National Animal Disease Center, Agricultural Research Service, USDA, 2300 Dayton Rd, PO Box 70, Ames, IA 50010 (Brogden, Cutlip, Lehmkuhl, Sacks) and H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, PO Box 280179, Tampa, FL 33682-0179 (Chedid).

Search for other papers by Kim A. Brogden in
Current site
Google Scholar
PubMed
Close
 PhD
,
Louis Chedid From the National Animal Disease Center, Agricultural Research Service, USDA, 2300 Dayton Rd, PO Box 70, Ames, IA 50010 (Brogden, Cutlip, Lehmkuhl, Sacks) and H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, PO Box 280179, Tampa, FL 33682-0179 (Chedid).

Search for other papers by Louis Chedid in
Current site
Google Scholar
PubMed
Close
 MD, PhD
,
Randall C. Cutlip From the National Animal Disease Center, Agricultural Research Service, USDA, 2300 Dayton Rd, PO Box 70, Ames, IA 50010 (Brogden, Cutlip, Lehmkuhl, Sacks) and H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, PO Box 280179, Tampa, FL 33682-0179 (Chedid).

Search for other papers by Randall C. Cutlip in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Howard D. Lehmkuhl From the National Animal Disease Center, Agricultural Research Service, USDA, 2300 Dayton Rd, PO Box 70, Ames, IA 50010 (Brogden, Cutlip, Lehmkuhl, Sacks) and H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, PO Box 280179, Tampa, FL 33682-0179 (Chedid).

Search for other papers by Howard D. Lehmkuhl in
Current site
Google Scholar
PubMed
Close
 PhD
, and
Jerome Sacks From the National Animal Disease Center, Agricultural Research Service, USDA, 2300 Dayton Rd, PO Box 70, Ames, IA 50010 (Brogden, Cutlip, Lehmkuhl, Sacks) and H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, PO Box 280179, Tampa, FL 33682-0179 (Chedid).

Search for other papers by Jerome Sacks in
Current site
Google Scholar
PubMed
Close
 PhD

Click on author name to view affiliation information

SUMMARY

Colostrum-deprived lambs and CF1 mice were vaccinated with water-in-oil emulsion vaccines containing nonviable whole cells (wc) of Corynebacterium pseudotuberculosis with and without muramyl dipeptide (mdp). Efficacy of vaccines was determined from the survival of mice and lesions in lambs after IV injection of 104 colony-forming units of C pseudotuberculosis. In mice, protection was related to the concentration of wc in the vaccine. At 50, 100, or 150 μg of wc, protection was good (78.8%). At 10 or 25 μg of wc, protection was considerably less (54.7%). At high wc concentrations, protection could only be moderately increased to 82.3% with high (50 and 100 μg) concentrations of mdp or increased to 90% protection with low (5 and 10 μg) concentrations of mdp. At low wc concentrations, protection significantly decreased to 32% (P < 0.025) with high concentrations of mdp, but significantly increased to 72.5% (P < 0.025) with low concen-trations of mdp. Therefore, the amount of protection with lower concentrations of wc and mdp was comparable with the amount of protection with higher concentrations of wc without mdp.

In lambs, high prechallenge antibody titers (geometric mean titers from 5.1 to 5.4 by day 35) were observed after vaccination with wc. Protection and vaccination site abscesses in lambs were related to the concentration of wc and mdp. Pulmonary or vaccination site abscesses were not observed in 4 of 4 lambs vaccinated with 1 mg of wc + 50 μg of mdp.

SUMMARY

Colostrum-deprived lambs and CF1 mice were vaccinated with water-in-oil emulsion vaccines containing nonviable whole cells (wc) of Corynebacterium pseudotuberculosis with and without muramyl dipeptide (mdp). Efficacy of vaccines was determined from the survival of mice and lesions in lambs after IV injection of 104 colony-forming units of C pseudotuberculosis. In mice, protection was related to the concentration of wc in the vaccine. At 50, 100, or 150 μg of wc, protection was good (78.8%). At 10 or 25 μg of wc, protection was considerably less (54.7%). At high wc concentrations, protection could only be moderately increased to 82.3% with high (50 and 100 μg) concentrations of mdp or increased to 90% protection with low (5 and 10 μg) concentrations of mdp. At low wc concentrations, protection significantly decreased to 32% (P < 0.025) with high concentrations of mdp, but significantly increased to 72.5% (P < 0.025) with low concen-trations of mdp. Therefore, the amount of protection with lower concentrations of wc and mdp was comparable with the amount of protection with higher concentrations of wc without mdp.

In lambs, high prechallenge antibody titers (geometric mean titers from 5.1 to 5.4 by day 35) were observed after vaccination with wc. Protection and vaccination site abscesses in lambs were related to the concentration of wc and mdp. Pulmonary or vaccination site abscesses were not observed in 4 of 4 lambs vaccinated with 1 mg of wc + 50 μg of mdp.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 113 113 64
PDF Downloads 32 32 1
Advertisement