Detection of capsular polysaccharide in milk cows of with natural intramammary infection caused by Staphylococcus aureus

Laurent Sutra From Station de Pathologie de la Reproduction, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.

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Bernard Poutrel From Station de Pathologie de la Reproduction, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.

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SUMMARY

Detection of capsular polysaccharide (cp) in milk of cows with natural intramammary infection caused by Staphylococcus aureus was attempted. Five quarters of 5 cows harboring S aureus strains that produce type-8 cp were selected. Using an elisa with a monoclonal antibody, type-8 cp was not detected in extracts prepared from fresh milk collected aseptically. By contrast, cp was easily detectable after incubation of infected milk at 38 C for 20 hours. Quantitation of cp in extracts from incubated milk samples by use of elisa indicated a great variation of cp expression by strains. Although an incubation step was necessary to detect cp, results of the study indicate that cp may be expressed in vivo during intramammary infection caused by S aureus.

SUMMARY

Detection of capsular polysaccharide (cp) in milk of cows with natural intramammary infection caused by Staphylococcus aureus was attempted. Five quarters of 5 cows harboring S aureus strains that produce type-8 cp were selected. Using an elisa with a monoclonal antibody, type-8 cp was not detected in extracts prepared from fresh milk collected aseptically. By contrast, cp was easily detectable after incubation of infected milk at 38 C for 20 hours. Quantitation of cp in extracts from incubated milk samples by use of elisa indicated a great variation of cp expression by strains. Although an incubation step was necessary to detect cp, results of the study indicate that cp may be expressed in vivo during intramammary infection caused by S aureus.

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