Frequency of association of noncytopathic bovine viral diarrhea virus with bovine neutrophils and mononuclear leukocytes before and after treatment with trypsin

Steven R. Bolin From the USDA, Agricultural Research Service, National Animal Disease Center, PO Box 70, Ames, IA 50010.

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 DVM, PhD
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Julia F. Ridpath From the USDA, Agricultural Research Service, National Animal Disease Center, PO Box 70, Ames, IA 50010.

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 PhD

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SUMMARY

Enriched populations of neutrophils and mononuclear leukocytes from 9 cattle persistently infected with non-cytopathic bovine viral diarrhea virus were analyzed for frequency of association with virus, using flow cytometric procedures. Trypsinization of neutrophils decreased the frequency of viral association from 0.82% to 0.49%. Similar treatment of mononuclear leukocytes decreased the frequency of viral association from 5.53% to 4.81%. Results of immunocytochemical procedures to locate viral antigen were inconclusive for neutrophils, but viral antigen was found in the cytoplasm of mononuclear leukocytes. A distinct and highly pure population of eosinophils was identified during flow cytometric analysis of neutrophil populations from 2 of 9 cattle.

SUMMARY

Enriched populations of neutrophils and mononuclear leukocytes from 9 cattle persistently infected with non-cytopathic bovine viral diarrhea virus were analyzed for frequency of association with virus, using flow cytometric procedures. Trypsinization of neutrophils decreased the frequency of viral association from 0.82% to 0.49%. Similar treatment of mononuclear leukocytes decreased the frequency of viral association from 5.53% to 4.81%. Results of immunocytochemical procedures to locate viral antigen were inconclusive for neutrophils, but viral antigen was found in the cytoplasm of mononuclear leukocytes. A distinct and highly pure population of eosinophils was identified during flow cytometric analysis of neutrophil populations from 2 of 9 cattle.

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