Modulation of arachidonic acid metabolism by bovine alveolar macrophages exposed to interferons and lipopolysaccharide

M. G. O'Sullivan From the Departments of Microbiology, Parasitology, and Pathology (O’Sullivan, MacLachlan, Brown) and Anatomy, Physiology, and Radiology (Fleisher, Olson), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by M. G. O'Sullivan in
Current site
Google Scholar
PubMed
Close
 MVB, PhD
,
L. N. Fleisher From the Departments of Microbiology, Parasitology, and Pathology (O’Sullivan, MacLachlan, Brown) and Anatomy, Physiology, and Radiology (Fleisher, Olson), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by L. N. Fleisher in
Current site
Google Scholar
PubMed
Close
 PhD
,
N. C. Olson From the Departments of Microbiology, Parasitology, and Pathology (O’Sullivan, MacLachlan, Brown) and Anatomy, Physiology, and Radiology (Fleisher, Olson), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by N. C. Olson in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
N. J. MacLachlan From the Departments of Microbiology, Parasitology, and Pathology (O’Sullivan, MacLachlan, Brown) and Anatomy, Physiology, and Radiology (Fleisher, Olson), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by N. J. MacLachlan in
Current site
Google Scholar
PubMed
Close
 BVSc, PhD
, and
T. T. Brown Jr. From the Departments of Microbiology, Parasitology, and Pathology (O’Sullivan, MacLachlan, Brown) and Anatomy, Physiology, and Radiology (Fleisher, Olson), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by T. T. Brown Jr. in
Current site
Google Scholar
PubMed
Close
 DVM, PhD

Click on author name to view affiliation information

SUMMARY

Stimulation of bovine alveolar macrophages with calcium ionophore A23187 resulted in marked production of leukotriene (lt)B4 and a lesser increase in thromboxane (tx)B2, whereas opsonized zymosan (opz) resulted in production of txb2 and relatively small increases in ltb4 and prostaglandin (pg)F. Alveolar macrophages incubated with recombinant bovine interferon-γ or lipopolysaccharide, and subsequently stimulated with A23187 or opz, had altered arachidonic acid metabolism, producing markedly increased amounts of txb2 and pgf, and slightly increased ltb4. Incubation of alveolar macrophages with lipopolysaccharide had a more profound effect on the increased amounts of txb2 and pgf, observed in response to stimulation with A23187 or opz, than did incubation with interferon-γ. Alveolar macrophages incubated with recombinant bovine interferon-α1-1 also produced slightly increased amounts of ltb4 when stimulated with A23187 or opz. Altered arachidonic acid metabolism by alveolar macrophages exposed to interferons and lipopolysaccharide may contribute to the development of pulmonary inflammation, such as in the early stages of bacterial pneumonia following viral infections that induce interferon production.

SUMMARY

Stimulation of bovine alveolar macrophages with calcium ionophore A23187 resulted in marked production of leukotriene (lt)B4 and a lesser increase in thromboxane (tx)B2, whereas opsonized zymosan (opz) resulted in production of txb2 and relatively small increases in ltb4 and prostaglandin (pg)F. Alveolar macrophages incubated with recombinant bovine interferon-γ or lipopolysaccharide, and subsequently stimulated with A23187 or opz, had altered arachidonic acid metabolism, producing markedly increased amounts of txb2 and pgf, and slightly increased ltb4. Incubation of alveolar macrophages with lipopolysaccharide had a more profound effect on the increased amounts of txb2 and pgf, observed in response to stimulation with A23187 or opz, than did incubation with interferon-γ. Alveolar macrophages incubated with recombinant bovine interferon-α1-1 also produced slightly increased amounts of ltb4 when stimulated with A23187 or opz. Altered arachidonic acid metabolism by alveolar macrophages exposed to interferons and lipopolysaccharide may contribute to the development of pulmonary inflammation, such as in the early stages of bacterial pneumonia following viral infections that induce interferon production.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 74 74 6
PDF Downloads 61 61 42
Advertisement