Recombinant DNA probe detecting Eperythrozoon suis in swine blood

R. D. Oberst From the Departments of Pathology (Oberst, Hall, Jasso, Arndt), College of Veterinary Medicine, and the Department of Biochemistry (Wen), Kansas State University, Manhattan, KS 66506.

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S. M. Hall From the Departments of Pathology (Oberst, Hall, Jasso, Arndt), College of Veterinary Medicine, and the Department of Biochemistry (Wen), Kansas State University, Manhattan, KS 66506.

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R. A. Jasso From the Departments of Pathology (Oberst, Hall, Jasso, Arndt), College of Veterinary Medicine, and the Department of Biochemistry (Wen), Kansas State University, Manhattan, KS 66506.

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T. Arndt From the Departments of Pathology (Oberst, Hall, Jasso, Arndt), College of Veterinary Medicine, and the Department of Biochemistry (Wen), Kansas State University, Manhattan, KS 66506.

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L. Wen From the Departments of Pathology (Oberst, Hall, Jasso, Arndt), College of Veterinary Medicine, and the Department of Biochemistry (Wen), Kansas State University, Manhattan, KS 66506.

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SUMMARY

A genomic library to Eperythrozoon suis dnawas constructed in λ gt11, and from this library, E suis clone KSU-2 was identified as a potential diagnostic probe. In hybridization experiments that used 100-μl samples of blood collected in chaotropic salt solutions, the KSU-2 probe hybridized strongly with purified E suis organisms and blood samples from splenectomized swine that were parasitized with E suis. However, the probe under stringent conditions did not give radiographic indications of hybridizing with equine blood dna, bovine blood dna infected with Anaplasma marginale, canine blood dna infected with Ehrlichia canis, feline blood dna infected with Haemobartonella felis, or uninfected swine blood dna.

SUMMARY

A genomic library to Eperythrozoon suis dnawas constructed in λ gt11, and from this library, E suis clone KSU-2 was identified as a potential diagnostic probe. In hybridization experiments that used 100-μl samples of blood collected in chaotropic salt solutions, the KSU-2 probe hybridized strongly with purified E suis organisms and blood samples from splenectomized swine that were parasitized with E suis. However, the probe under stringent conditions did not give radiographic indications of hybridizing with equine blood dna, bovine blood dna infected with Anaplasma marginale, canine blood dna infected with Ehrlichia canis, feline blood dna infected with Haemobartonella felis, or uninfected swine blood dna.

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