Recombinant DNA probe detecting Eperythrozoon suis in swine blood

R. D. Oberst From the Departments of Pathology (Oberst, Hall, Jasso, Arndt), College of Veterinary Medicine, and the Department of Biochemistry (Wen), Kansas State University, Manhattan, KS 66506.

Search for other papers by R. D. Oberst in
Current site
Google Scholar
PubMed Close
 DVM, PhD
,
S. M. Hall From the Departments of Pathology (Oberst, Hall, Jasso, Arndt), College of Veterinary Medicine, and the Department of Biochemistry (Wen), Kansas State University, Manhattan, KS 66506.

Search for other papers by S. M. Hall in
Current site
Google Scholar
PubMed Close
 DVM, PhD
,
R. A. Jasso From the Departments of Pathology (Oberst, Hall, Jasso, Arndt), College of Veterinary Medicine, and the Department of Biochemistry (Wen), Kansas State University, Manhattan, KS 66506.

Search for other papers by R. A. Jasso in
Current site
Google Scholar
PubMed Close
 BS
,
T. Arndt From the Departments of Pathology (Oberst, Hall, Jasso, Arndt), College of Veterinary Medicine, and the Department of Biochemistry (Wen), Kansas State University, Manhattan, KS 66506.

Search for other papers by T. Arndt in
Current site
Google Scholar
PubMed Close
 BS
, and
L. Wen From the Departments of Pathology (Oberst, Hall, Jasso, Arndt), College of Veterinary Medicine, and the Department of Biochemistry (Wen), Kansas State University, Manhattan, KS 66506.

Search for other papers by L. Wen in
Current site
Google Scholar
PubMed Close
 PhD
Free access

SUMMARY

A genomic library to Eperythrozoon suis dnawas constructed in λ gt11, and from this library, E suis clone KSU-2 was identified as a potential diagnostic probe. In hybridization experiments that used 100-μl samples of blood collected in chaotropic salt solutions, the KSU-2 probe hybridized strongly with purified E suis organisms and blood samples from splenectomized swine that were parasitized with E suis. However, the probe under stringent conditions did not give radiographic indications of hybridizing with equine blood dna, bovine blood dna infected with Anaplasma marginale, canine blood dna infected with Ehrlichia canis, feline blood dna infected with Haemobartonella felis, or uninfected swine blood dna.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 3490 3427 1
PDF Downloads 50 25 0
Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Issue:
01 Nov 1990
Advertisement