Method for obtaining bovine zygotes produced in vivo

Joanna E. Ellington From the Department of Animal Science, New York State College of Agriculture and Life Sciences, 202 Morrison Hall, Cornell University, Ithaca, NY 14853-4801.

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Paul B. Farrell From the Department of Animal Science, New York State College of Agriculture and Life Sciences, 202 Morrison Hall, Cornell University, Ithaca, NY 14853-4801.

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Michael E. Simkin From the Department of Animal Science, New York State College of Agriculture and Life Sciences, 202 Morrison Hall, Cornell University, Ithaca, NY 14853-4801.

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Robert H. Foote From the Department of Animal Science, New York State College of Agriculture and Life Sciences, 202 Morrison Hall, Cornell University, Ithaca, NY 14853-4801.

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 PhD

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SUMMARY

A superovulatory and surgical protocol was developed for recovery of bovine zygotes. Holstein cows and heifers were given follicle-stimulating hormone and cloprostenol to induce superovulation. Surgical cannulation and lavage of the uterine tube was performed 40 to 48 hours after the start of standing estrus. In general, cows had more corpora hemorrhagica than did heifers, but a higher percentage (P < 0.05) of ova recovered from cows were infertile. Several heifers were subjected to the procedure twice, and embryo recovery rates were equivalent both times.

SUMMARY

A superovulatory and surgical protocol was developed for recovery of bovine zygotes. Holstein cows and heifers were given follicle-stimulating hormone and cloprostenol to induce superovulation. Surgical cannulation and lavage of the uterine tube was performed 40 to 48 hours after the start of standing estrus. In general, cows had more corpora hemorrhagica than did heifers, but a higher percentage (P < 0.05) of ova recovered from cows were infertile. Several heifers were subjected to the procedure twice, and embryo recovery rates were equivalent both times.

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