SUMMARY
Cellular and humoral immune responses of pigs inoculated with Mycoplasma hyopneumoniae were investigated at postinoculation weeks (piw) 2, 4, and 6. The response of blood lymphocytes (bl) and bronchial lymph node lymphocytes (lnl) to stimulation by M hyopneumoniae antigens was evaluated by a lymphocyte-stimulation test. Specific antibodies in serum and lung washing samples were assayed by elisa. Immunoglobulin-positive cells in lungs and bronchial lymph nodes were identified by indirect fluorescent antibody test, using isotype-specific monoclonal antibodies.
At piw 0 to 6, bl from control and M hyopneumoniae-inoculated pigs were stimulated by M hyopneumoniae cells; however, bl from inoculated pigs generally had higher stimulation indices, especially at piw 6. The response of lnl was influenced by previous exposure to M hyopneumoniae, as indicated by higher stimulation indices (P < 0.01) of lnl from inoculated pigs killed at piw 2 and 6. Specific elisa antibodies to M hyopneumoniae in lung washings from inoculated pigs consisted mainly of IgG and IgA isotypes.
Examination of lung sections by indirect immunofluorescence revealed that cells producing IgM and IgA were in controls as well as M hyopneumoniae-inoculated pigs, but IgG-positive cells were only in lungs of inoculated pigs. Resolution of pneumonia appeared to correlate with development of increased sensitization of bl, as well as development of marked increases in immunoglobulins, particularly IgG in lung washings at piw 6.
