Editorial Type:
Immunology

Biological reactivity of Moraxella bovis lipopolysaccharide

K. A. Johansen From the Veterinary Medical Research Institute, Iowa State University, Ames, IA 50011 (Wannemuehler, Rosenbusch) and the Department of Medical Microbiology, University of Missouri, Columbia, MO 65201 (Johansen).

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 MS
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M. J. Wannemuehler From the Veterinary Medical Research Institute, Iowa State University, Ames, IA 50011 (Wannemuehler, Rosenbusch) and the Department of Medical Microbiology, University of Missouri, Columbia, MO 65201 (Johansen).

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 PhD
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R. F. Rosenbusch From the Veterinary Medical Research Institute, Iowa State University, Ames, IA 50011 (Wannemuehler, Rosenbusch) and the Department of Medical Microbiology, University of Missouri, Columbia, MO 65201 (Johansen).

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 DVM, PhD
DOI:
https://doi.org/10.2460/ajvr.1990.51.01.46
Volume/Issue:
Volume 51: Issue 1
Received:
17 Oct 1988
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Online Publication Date:
01 Jan 1990
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SUMMARY

Lipopolysaccharide (lps) was isolated from Moraxella bovis 118F and ATCC 10900, M ovis ATCC 33078, and M phenylpyruvica ATCC 23333 by hot phenol-water extraction. In silver-stained sodium dodecyl sulfate polyacrylamide electrophoresis gels, M bovis 118F lps had a smooth profile, whereas the other Moraxella preparations appeared to be rough. The lps preparations induced pyrogenicity and dermal Shwartzman reactions in rabbits, and induced production of tumor necrosis factor and interleukin- 1 in vitro. Induction of tumor necrosis factor appeared to be among the most potent biological activities of M bovis lps.

SUMMARY

Lipopolysaccharide (lps) was isolated from Moraxella bovis 118F and ATCC 10900, M ovis ATCC 33078, and M phenylpyruvica ATCC 23333 by hot phenol-water extraction. In silver-stained sodium dodecyl sulfate polyacrylamide electrophoresis gels, M bovis 118F lps had a smooth profile, whereas the other Moraxella preparations appeared to be rough. The lps preparations induced pyrogenicity and dermal Shwartzman reactions in rabbits, and induced production of tumor necrosis factor and interleukin- 1 in vitro. Induction of tumor necrosis factor appeared to be among the most potent biological activities of M bovis lps.

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Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Jan 1990
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