Plasmid content of piliated and nonpiliated forms of Moraxella bovis

G. R. Wilt From the Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519.

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Gin Wu From the Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519.

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R. C. Bird From the Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519.

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M. Toivio-Kinnucan From the Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519.

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SUMMARY

Plasmid profiles were compared between nonpiliated and piliated forms of Moraxella bovis isolates. The piliated form of M bovis isolate IBH64 contained 1 fewer plasmid than did the nonpiliated form. Piliated and nonpiliated cells of IBH64 contained plasmids having molecular size of 45, 32.8, 4.9, and 4.6 kilobases (kb). Single- and double-restriction endonuclease digestion by Ava I and Nde I indicated that the size of the additional plasmid carried by the nonpiliated form of IBH64 was approximately 43.6 kb. The M bovis isolates, Newport and GRS, contained the same number of plasmids in either their piliated or nonpiliated form.

SUMMARY

Plasmid profiles were compared between nonpiliated and piliated forms of Moraxella bovis isolates. The piliated form of M bovis isolate IBH64 contained 1 fewer plasmid than did the nonpiliated form. Piliated and nonpiliated cells of IBH64 contained plasmids having molecular size of 45, 32.8, 4.9, and 4.6 kilobases (kb). Single- and double-restriction endonuclease digestion by Ava I and Nde I indicated that the size of the additional plasmid carried by the nonpiliated form of IBH64 was approximately 43.6 kb. The M bovis isolates, Newport and GRS, contained the same number of plasmids in either their piliated or nonpiliated form.

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