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Introduction Weight estimation has a rich history within Americana, and its importance is far-reaching: from turn-of-the-century oxen estimates, 1 to midway carnival barkers estimating customers’ weights, 2 to health-conscious political

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in Journal of the American Veterinary Medical Association

inaccurately calculated. While drug and fluid doses should always be based on measured weight, patient weight estimates are required when a weight cannot be quickly obtained. Thus, accurate weight estimation is critical for calculating drug doses, determining

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in Journal of the American Veterinary Medical Association

Abstract

Objective—To purify and partially characterize various isoforms of canine pepsinogen (PG) from gastric mucosa.

Sample Population—Stomachs obtained from 6 euthanatized dogs.

Procedure—Mucosa was scraped from canine stomachs, and a crude mucosal extract was prepared and further purified by use of weak anion-exchange chromatography, hydroxyapatite chromatography, sizeexclusion chromatography, and strong anionexchange chromatography. Pepsinogens were characterized by estimation of molecular weights, estimation of their isoelectric points (IEPs), and N-terminal amino acid sequencing.

Results—Two different groups of canine PG were identified after the final strong anion-exchange chromatography: PG A and PG B. Pepsinogens differed in their molecular weights and IEP. Pepsinogen B appeared to be a dimer with a molecular weight of approximately 34,100 and an IEP of 4.9. Pepsinogen A separated into several isoforms. Molecular weights for the various isoforms of PG A ranged from 34,200 to 42,100, and their IEPs ranged from 4.0 to < 3.0. The N-terminal amino acid sequence for the first 25 amino acid residues for PG A and B had good homology with the amino acid sequences for these proteins in other species.

Conclusions and Clinical Relevance—Canine PG B and several isoforms of canine PG A have been purified. Availability of these PGs will facilitate development of immunoassays to measure PG in canine serum as a potential diagnostic marker for gastric disorders in dogs. (Am J Vet Res 2002;63:1585–1590)

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in American Journal of Veterinary Research

determined at the time of enrollment with a chest girth weight estimation tape. 10,11 A dosing table specified the horse's weight range for each 0.25-mL increment of the dose. A veterinarian, a technician, or the horse's owner or agent administered the dose

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in Journal of the American Veterinary Medical Association

the first recorded weight or after the last recorded weight. Interpolation was not possible when weight was not recorded for a dog at any visit. For that situation, a weight estimation procedure based on the mean weight for dogs with the same age

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in Journal of the American Veterinary Medical Association

. 2. Carroll CL , Huntington PJ . Body condition and weight estimation of horses . Equine Vet J 1988 ; 20 : 41 – 45 . 10.1111/j.2042-3306.1988.tb01451.x 3. Argenta FF , de Souza SO , Meirelles LS , Histiocytic ulcerative

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in Journal of the American Veterinary Medical Association

tape and weight estimation was determined by 1 of 2 people throughout the study. Horses from both groups received detomidine hydrochloride (0.02 mg/kg) intravenously (IV) as premedication. Detomidine was used for its higher affinity as an alpha-2

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in American Journal of Veterinary Research

, Effects of topically instilled drugs on intraocular pressure in rabbits . Arzneimittelforschung 1975 ; 25 : 806 – 809 . 9. Carroll CL , Huntington PJ . Body condition scoring and weight estimation of horses . Equine Vet J 1988 ; 20 : 41 – 45

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in American Journal of Veterinary Research

. Carroll CL , Huntington PJ . Body condition scoring and weight estimation of horses . Equine Vet J 1988 ; 20 : 41 – 45 . 10.1111/j.2042-3306.1988.tb01451.x 2. Keggan A , Freer H , Rollins A , et al. Production of seven monoclonal

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in Journal of the American Veterinary Medical Association

weight estimation of horses . Equine Vet J 1988 ; 20 : 41 – 45 . 10.1111/j.2042-3306.1988.tb01451.x 13. Muir WW Wade A Grospitch B . Automatic noninvasive sphygmomanometry in horses . J Am Vet Med Assoc 1983 ; 182 : 1230 – 1233 . 14

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in American Journal of Veterinary Research