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hemorrhage, edema, and fibrin with marked neutrophils. H&E stain; bar = 20 µm. Other clinically important histologic findings included a similar necrohemorrhagic pleuritis, pericarditis, pneumonia, lymphadenitis, and myositis. There was widespread

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in Journal of the American Veterinary Medical Association

Masticatory myositis is an autoimmune disease affecting the muscles of mastication in dogs. Biochemical and histochemical differences between the myofibers of masticatory and limb muscles provide the basis for the selective targeting of

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in Journal of the American Veterinary Medical Association

substantial muscle damage or necrosis in this sea lion. Differential diagnoses for myositis in this sea lion included infectious disease, nutritional deficiency, exertional myopathy, immune-mediated myositis, toxicosis, hypothyroidism, and hyperadrenocorticism

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in Journal of the American Veterinary Medical Association

SUMMARY

Sarcocystis cruzi sarcocysts were isolated from eosinophilic myositis (em)-affected and nonaffected bovine hearts. Isolates were ruptured and used to prepare a bradyzoite antigen extract from each heart. The nonaffected heart from one newborn calf contained no apparent sarcocysts when examined histologically and was used to prepare Sarcocystis-negative control antigen. Blood samples were taken from the heart approximately 20 minutes after slaughter. Serum was obtained and evaluated, using a radioimmunoassay to measure Sarcocystis-specific IgG and IgE titers. Sarcocystis cruzi extract from a heart without em lesions was used for antigen in the radioimmunoassay. Sarcocystis-specific IgG titer ranged between 1:1,280 and 1:2, 560 in em-affected cattle and was 1:640 in nonaffected cattle. Sarcocystis-specific IgE titer ranged between 1:640 and 1:1,280 in em-affected and nonaffected cattle.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and protein (western) immunoblot analysis were used to compare antigen extracts and serum samples from em-affected vs nonaffected cattle. Twenty protein bands, ranging from approximately 22 to 215 kD, were detected consistently on bradyzoite blots probed with anti-bovine IgG after incubation with serum samples. Seven of these bands, 37, 44, 53, 57, 94, 113, and 215 kD, were also detected consistently on bradyzoite blots probed with monoclonal anti-bovine IgE. One additional band, 61 kD, was detected consistently on bradyzoite blots probed for IgE, but was seldom recognized when probed for IgG. Sixteen protein bands were evident in silver-stained gels of S cruzi-negative, newborn calf antigen, but none were recognized by antisera on western blots. Consistent differences were not found among antigen extracts or among serum from em-affected vs nonaffected cattle on silver-stained gels or western blots.

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in American Journal of Veterinary Research
in Journal of the American Veterinary Medical Association

mononuclear cell infiltrations were evident under the perineurium and within the endoneurium in the ulnar nerve biopsy specimen ( Figure 2 ). On the basis of these findings, a diagnosis of inflammatory myopathy (myositis) and neuropathy (neuritis) was made

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in Journal of the American Veterinary Medical Association

and macrophage infiltrate and occasional plump fibroblasts. The microscopic interpretation was consistent with mild to moderate multifocal neutrophilic and histiocytic myositis with myofiber degeneration and necrosis ( Figure 4 ). Figure 4

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in Journal of the American Veterinary Medical Association

-differentiated adipose tissue with infiltrated neutrophils and macrophages and scattered bleedings. Histologic examination of the spinous process revealed a normal bony structure. These findings confirmed a diagnosis of suppurative myositis and suppurative epidural

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in Journal of the American Veterinary Medical Association