Search Results

You are looking at 1 - 10 of 89 items for :

  • "multidrug resistance" x
  • Refine by Access: All Content x
Clear All

main reason for treatment failure. 1–4 Multidrug resistance–associated proteins are a group of ATP-dependent plasma membrane transporters responsible for cellular drug efflux. The cellular membrane location of these pumps in normal cells suggests an

Full access
in American Journal of Veterinary Research

identified as a drug-resistance gene that conferred multidrug resistance on tumor cells. The mdr1 encodes P-gp, a transmembrane protein responsible for efflux of various substrates in an energy-dependent manner driven by ATP. 1 The P-gp effluxes the

Full access
in American Journal of Veterinary Research

resonance energy transfer MDR Multidrug resistance MIC Minimum inhibitory concentration MPC Mutation prevention concentration PAβN Phe-Arg-β-naphthylamide QRDR Quinolone resistance-determining region RND Resistance-nodulation-division a

Full access
in American Journal of Veterinary Research

Abstract

Objective—To evaluate the mechanism of multidrug resistance in feline lymphoma cell lines.

Sample Population—A feline lymphoma cell line (FT-1) and its adriamycin (ADM)-resistant subline (FT-1/ADM).

Procedures—The FT-1 cell line was cultivated in the presence of a gradually increasing concentration of ADM to generate its ADM-resistant subline (FT-1/ADM). Susceptibility of cells from the parental FT-1 cell line and the FT-1/ADM subline to antineoplastic drugs was determined. From the complementary DNA (cDNA) template of FT-1/ADM cells, feline MDR1 cDNA was amplified by use of polymerase chain reaction (PCR) and sequenced. Reverse transcription (RT)-PCR and Western blot analyses were performed to assess expression of the MDR1 gene and P-glycoprotein (P-gp) in FT-1/ADM cells, compared with that in FT-1 cells.

Results—A drug sensitivity assay revealed that FT-1/ADM cells were much more resistant to ADM and vincristine than the parental FT-1 cells. The feline MDR1 cDNA amplified by use of PCR was 3,489 base pairs long, corresponding to approximately 90% of the whole open reading frame of human MDR1 cDNA; its amino acid sequence was 91.5, 87.0, and 79.4% identical to that of human MDR1, mouse mdr1a, and mdr1b cDNA, respectively. By RT-PCR analysis, expression of MDR1 messenger RNA was clearly detected in FT-1/ADM cells but not in the parental FT-1 cells. Western blot analysis also revealed the expression of P-gp encoded by the MDR1 gene in FT-1/ADM cells but not in FT-1 cells.

Conclusions—The basic structure of the feline MDR1 gene was essentially the same as that of multidrug- resistance genes of other species. Expression of P-gp appeared to be one of the mechanisms responsible for the development of multidrug resistance in feline lymphoma cell lines in vitro. (Am J Vet Res 2000;61:1122–1127)

Full access
in American Journal of Veterinary Research

and 18% are not susceptible to cefovecin. Moreover, E coli exhibits the capacity to acquire and accumulate resistance genes from other bacteria, resulting in multidrug resistance (MDR). 13 In companion animals, AMR and MDR E coli infections pose

Open access
in American Journal of Veterinary Research

; 246 ( 7 ): 785 - 793 . doi: 10.2460/javma.246.7.785 27. Motta RG , de Souza Araújo Martins L , da Silva RC , Etiology, multidrug resistance, and acute-phase proteins biomarkers as in equine septic arthritis . Cienc Rural . 2020 ; 50

Open access
in Journal of the American Veterinary Medical Association

resistant by testing laboratories that use those guidelines. 21 Multidrug resistance has been associated with MRSA in humans and other animals 8,22 and has been reported in S intermedius in the absence of oxacillin resistance. 3,13,23 The recent

Full access
in Journal of the American Veterinary Medical Association

surgeries, bone or tendon involvement, multiple infected synovial structures, treatment with doxycycline, and infections with gram-negative bacteria, particularly those with multidrug resistance (MDR). 1 – 6 Performance-limiting complications include

Open access
in American Journal of Veterinary Research

dog described in the present report, including when and where the organisms were acquired, is unclear. The dog received multiple courses of β-lactam antimicrobials, to which MRSP is resistant, which could have facilitated development of multidrug

Full access
in Journal of the American Veterinary Medical Association

Epidemiology of Methicillin-Resistant Staphylococci Definitions of methicillin and multidrug resistance The antibacterial drug methicillin was first introduced in 1959, and at the time it was hoped that it would be the end of

Full access
in Journal of the American Veterinary Medical Association