Search Results

You are looking at 1 - 10 of 120 items for :

  • "mitochondria" x
  • Refine by Access: All Content x
Clear All

culmination of genetic alterations derived from selective breeding. With regards to the WB myopathy, a major area of research interest has been with mitochondria function 5 and energy production (ATP). 6 Mitochondrial abnormalities are responsible for

Open access
in American Journal of Veterinary Research

mitochondrial genes as well as transcription factor A mitochondrial, 6 a nuclear-encoded gene that controls mitochondrial gene transcription and copy number. 7 The mitochondria of affected Miniature Schnauzers had altered morphology and were reduced in size

Full access
in American Journal of Veterinary Research

-associated molecular patterns that have been well studied in humans and mice include HMGB1, heat shock proteins, nucleic acids, and S100 proteins. 3,5,11 The importance of DAMPs derived from the breakdown of mitochondria has more recently been recognized

Full access
in American Journal of Veterinary Research

Summary

Ultrastructure of the interactions of host cell mitochondria with developing Toxoplasma gondii tissue cysts was examined in cultured cells, using transmission electron microscopy of infected cells and rhodamine 123 (a mitochondria-specific vital fluorescent dye) staining of isolated tissue cysts. Structurally mature T gondii tissue cysts were observed as early as 2 days after inoculation of cultured cells. During development of T gondii, host cell mitochondria were observed surrounding the parasitophorous vacuole membrane. Mitochondria became flat and elongated in the vicinity of the parasitophorous vacuole membrane. These mitochondria were also closely associated with T gondii tissue cysts. Incubation of tissue cysts from cultured cells and tissue cysts from mouse brains with rhodamine 123 revealed fluorescence of the tissue cyst wall in living specimens. Incubation of tissue cysts with 10 µM rotenone caused diminished fluorescence of the tissue cyst walls, and 100 µM rotenone caused complete inhibition. Mouse RBC, and tissue cysts fixed in 100% methanol did not fluoresce after exposure to rhodamine. Tissue cysts in 9 isolates of T gondii from mouse brains were examined, using rhodamine 123, and the tissue cyst walls of all isolates fluoresced, indicating no isolate effects. Our results indicate that host cell mitochondria may be closely associated with the tissue cysts of T gondii in cell cultures and in mice.

Free access
in American Journal of Veterinary Research

Mitochondria generate most of the cellular energy, primarily through oxidative phosphorylation. Within the mitochondrial inner membrane, electrons pass through a series of carrier molecules known as the ETC. 1 The ETC together with H

Full access
in American Journal of Veterinary Research

essential for the transport of fatty acids into mitochondria for subsequent B-oxidation and plays an important role in the energy metabolism of skeletal muscle. Decreases in skeletal muscle carnitine content, as well as increased urinary carnitine excretion

Full access
in American Journal of Veterinary Research

-transfected MSC more often had a nucleolus. At low magnification, AT-MSCs and BM-MSCs appeared similar, in that each MSC had an irregular nucleus containing chromatin within the perinuclear cisternae. Well-developed organelles, including mitochondria, endoplasmic

Full access
in American Journal of Veterinary Research

energy source and source of additional ketones; taurine and the carnitine precursor amino acids, methionine and lysine, to support mitochondrial and other metabolic functions; the mineral magnesium to support cardiac mitochondria and other functions; and

Full access
in Journal of the American Veterinary Medical Association

, recent findings 4 , 5 in molecular biology stressed that EVs may also become a cargo for functional cell organelles like mitochondria, endoplasmic reticulum, and ribosomes. In addition to variations due to cell type, the specific cargo within EVs may

Open access
in Journal of the American Veterinary Medical Association

fibers stained positive) and succinate dehydrogenase stains of sacrocaudalis dorsalis medialis muscle, confirming that the abnormal NADH-Tr staining involved mitochondria. Figure 2— Photomicrographs of cross-sections of sacrocaudalis dorsalis

Full access
in Journal of the American Veterinary Medical Association