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including the use of multilayered flasks, 3-D scaffold systems, and 3-D microcarrier-based systems. 8 Microcarriers provide an increased surface area, are very effective for adherent-cell culture, and can be used in dynamic culture systems, including

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in Journal of the American Veterinary Medical Association
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authors describe EV production using a 3-D culture of equine bone marrow–derived MSCs . The study found that 3-D culture of MSCs on microcarrier beads did not improve overall MSC expansion but did yield more EVs produced per cell. The authors provide

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in Journal of the American Veterinary Medical Association

microcarrier spinner cultures by the combination of avocado soybean unsaponifiables, chondroitin sulfate, and glucosamine (abstr). 34th Annu Vet Orthop Soc Conf 2007;25. y. Punke JP, Au RY, Au AY, et al. Modulation of prostaglandin E2 production in feline

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in American Journal of Veterinary Research