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preliminary experiment, we investigated whether Mx protein expression was detectable in CCs via immunohistochemistry involving murine anti-Mx protein MAb M143. In this experiment, Mx protein was detected in CCs from cats with naturally occurring herpes virus
immunohistochemistry in paraffin-embedded tissues . Vet Immunol Immunopathol 2004 ; 99 : 203 – 213 . 10.1016/j.vetimm.2004.02.001 22. Wiener G , Han JL , Long RJ . The yak . 2nd ed. Bangkok, Thailand : Regional Office for Asia and the Pacific Food
Instruments International Corp, Columbus, Ohio. f. Histology/Immunohistochemistry Lab, Comparative Pathology and Mouse Phenotyping Shared Resource, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio. g. Proc Mixed, SAS, version 9
, moderate chondroid metaplasia, and loss of collagen fiber bundling), or grade 3 (severe degenerative changes including large areas with metaplastic chondrocytes, mineralization, and fragmented or separated collagen fibers). Caspase-3 immunohistochemistry
primary chondrocytes suggest that MMP-13, but neither IL-1α nor IL-1β, is increased with osteoarthritis. In 1 study 17 of early osteoarthritis, mRNA expression for IL-1 and MMP-13 genes was increased. Similarly, by use of immunohistochemistry
appeared clinically normal 1 day earlier. All tissues, except for lymphoid tissue of the third eyelid, tested positive for PrP SC via IHC. Table 2— Immunohistochemistry test results for detection of PrP SC performed on lymphoid and brain tissue from
through effects on calf death, the occurrence of calf treatment, and calf weaning weight. ABBREVIATIONS BVDV Bovine viral diarrhea virus CI Confidence interval IBR Infectious bovine rhinotracheitis IHC Immunohistochemistry OR Odds ratio
of 1 horse/1 time/1 site were evaluated, and the most severe infiltration score was used for analysis. Immunohistochemistry and histomorphometry Immunohistochemistry was performed on all biopsies to quantify T lymphocytes (CD3) and B
Abstract
Objective
To examine the site-specific and dose-dependent effects of insulin-like growth factor I (IGF-I) on normal equine tendon in vitro.
Samples
Superficial digital flexor tendon explants derived from a euthanatized 3-year-old horse.
Procedure
Explants in culture were treated with 0, 100, 250, or 500 ng of IGF-I/ml for 14 days with an end-stage radiolabel of 20 μCi of [3H]proline/ml or 5 μCi of [3H]thymidine/ml. The tendon tissues were then analyzed biochemically for hydroxyproline content by reverse-phase high-performance liquid chromatography, DNA content by fluorometry, and glycosaminoglycan content by the dimethylmethylene blue dye-binding assay. In addition, morphologic analysis of the explants comprised histologic examination, autoradiography, and immunohistochemistry.
Results
Hydroxyproline content was significantly increased in explants treated with 100 and 250 ng of IGF-I/ml. Additionally, the collagen synthetic rate, measured by incorporation of [3H]proline into hydroxyproline, was significantly increased for all treatment groups. On the basis of autoradiograms, fibroblast proliferation and collagen synthesis were predominantly confined to the endcap and adjacent endotenon of the explants. Enhanced immunoreactivity for type-I collagen, compared with type-III collagen, was evident in the treated explants, an observation supported by positive staining for type-I collagen with picrosirius red. Histologically, treated explants contained greater numbers of larger and more metabolically active fibroblasts, compared with untreated controls.
Conclusion
IGF-I enhances collagen synthesis in normal equine flexor tendon in a dose-dependent manner. IGF-I also exerts its primary effect on cell proliferation and collagen synthesis in the epitenon and adjacent endotenon and accompanying perivascular connective tissues, consistent with enhancement of intrinsic tendon metabolism.
Clinical Relevance
IGF-I may have a potential role in the treatment of tendinitis in horses. (Am J Vet Res 1997;58:103–109)
guest author Amy L. Warren, describes some immunologic-based clinical laboratory tests such as those used to detect antibody-antigen reactions (eg, ELISA, immunoblotting, immunofluorescent microscopy, and immunohistochemistry), blood type