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has focused on in vitro elution. 8 – 12 In vitro elution carboplatin studies have mostly been performed in PBS including release from poloxamer 407 8 and release into a gelatin block to mimic tissue. 13 Elution studies in plasma might more closely

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in American Journal of Veterinary Research

, and duration of elution of AgNPs from such a construct could be used to guide clinical recommendations for the integration of nanotechnology into clinical practice and treatment of MDR infections. The objectives of the study reported here were to

Open access
in American Journal of Veterinary Research

to adjust the elution behavior of POP and enhance bone formation in infected tissues. The objective of the study reported here was to evaluate the effects of SIS on the elution characteristics of gentamicin from POP pellets. We hypothesized that SIS

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in American Journal of Veterinary Research

, 12 The combination of these factors makes systemic administration of antimicrobials alone an inefficient—and in some cases, ineffective—treatment option. 13 When implanted into a surgical wound, AIMs demonstrate sustained elution of the

Open access
in American Journal of Veterinary Research

published data exist regarding the rate, pattern, and duration of elution of platinum from commercially available carboplatin-impregnated CSH beads. The purpose of the study reported here was to evaluate whether platinum elutes from carboplatin

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in American Journal of Veterinary Research

carriers. Previously, we have described the short-term elution characteristics of CI-CSH beads in an in vitro study. 21 Elution is the practice of extracting one material from another by washing it with a solvent. Historically, this process has been used

Full access
in American Journal of Veterinary Research

SUMMARY

Objective

To develop a high-performance liquid chromatography (HPLC) assay for insulin-like growth factor I (IGF-I) and to use it to quantify elution of IGF-I from polymerized fibrin in an in vitro system.

Sample Population

Equine fibrinogen and calcium-activated bovine thrombin were used to form fibrin containing human recombinant IGF-I.

Procedure

Multiple fibrin disks were formed from polymerized fibrinogen and thrombin; 6 disks were loaded with 25 μg of recombinant human IGF-I at the time of polymerization, and 4 remained as unladen controls. The resultant clots were incubated at 37 C and 90% humidity for 22 days. Phosphate-buffered saline solution in each well was replaced daily, and IGF-I content was assayed by HPLC. Solid-phase separation was used to assay free IGF-I peptide peaks. A scan was done to determine optimal wavelength for IGF-I absorbance. Commercially pure IGF-I was used to construct a standard curve, and the IGF-I content of medium removed from all 10 wells each day was assayed.

Results

Pure unbound IGF-I eluted from the fibrin polymers for 22 days; initial rapid daily release of 1.6 to 1.7 μg of IGF/ml of medium changed after day 3 to commence an asymptotic decrease to 110 ng of IGF/ml by day 22. The fibrin disks had dissolved by day 22, and the experiment was terminated. Control disks did not have detectable IGF-I content at any time. Limit of the HPLC assay was 25 ng of IGF-l/ml. Retention time for nonprotein-bound IGF-I was 10.3 ± 0.15 minutes.

Conclusion

IGF-I (25 μg) can be added to polymerized fibrin and eluted as free ligand over a 22-day period of culture at 37 C. Release of IGF-I was initially independent of fibrin dissolution, but later appeared to follow a pattern consistent with fibrin degradation.

Clinical Relevance

IGF-I can be incorporated as a depot form in polymerized fibrin and is released over time in sufficient concentration to effectively stimulate articular chondrocyte metabolic activity. (Am J Vet Res 1997;58:1431–1435)

Free access
in American Journal of Veterinary Research

retains efficacy against common multidrug-resistant bacteria, but its cost typically makes systemic use in horses prohibitive. Local delivery of antimicrobials such as meropenem by elution from AI-PMMA beads can provide a high concentration of the

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in American Journal of Veterinary Research

6.5-hour period to ensure that no bubbles of carbon dioxide formed and that the polycaprolactone coating remained intact. In vitro evaluation of BMP-2 elution from polycaprolactone–BMP-2 coating on bone screws —Four 3.5-mm-diameter, 316L, stainless

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in American Journal of Veterinary Research

methoxysuccinyl-alanine-alanine-proline-valine- p -nitroanilide, i a synthetic chromogenic substrate, were pooled and concentrated by use of a centrifugal filter device j with a molecular mass cutoff of 10 kd. Continuous elution electrophoresis —The

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in American Journal of Veterinary Research