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pancreatic lipase immunoreactivity. cTLI = Canine trypsin-like immunoreactivity. EPI = Exocrine pancreatic insufficiency. Statistical analyses were performed using R CRAN Statistical Software (R Core Team 9 ). Because all variables of interest did not

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in American Journal of Veterinary Research

(both acute and chronic) in dogs. Abbreviations CCK Cholecystokinin cPLI Canine pancreatic lipase immunoreactivity cTLI Canine trypsin–like immunoreactivity CV Coefficient of variation MCT Medium-chain triglyceride a. Steiner

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in American Journal of Veterinary Research

declare that there were no conflicts of interest. Abbreviations cPLI Canine pancreatic lipase immunoreactivity cTLI Canine trypsin-like immunoreactivity EPI Exocrine pancreatic insufficiency RER Resting energy requirement RI Reference

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in American Journal of Veterinary Research

Abstract

Objective—To assess the safety of endoscopic retrograde pancreatography (ERP) in dogs by performing repeated clinical examinations and laboratory analyses of serum amylase, lipase, canine trypsin-like immunoreactivity (cTLI), and canine pancreatic elastase 1 (cE1) after the procedure.

Animals—7 healthy Beagles.

Procedure—Clinical examinations were performed and blood samples obtained for serum enzyme determinations before and at intervals (10 minutes; 2, 4, and 6 hours; and 1, 2, and 3 days) after ERP.

Results—Repeated clinical examinations revealed no signs of ERP-induced complications in the 7 dogs. Results of repeated laboratory tests indicated a transient increase in serum values of amylase, lipase, and cTLI but not cE1. Mean ± SD lipase activity increased from 120.7 ± 116.4 U/L to 423.4 ± 243.1 U/L at 4 hours after ERP. Median serum cTLI concentration increased from 16.2 µg/L (range, 7.7 to 26.5 µg/L) to 34.9 µg/L (range, 16.6 to 68.3 µg/L) 10 minutes after ERP. Enzyme values returned to baseline levels at the latest on day 2 in 6 of 7 dogs. Highest values for serum amylase, lipase, and cTLI and their delayed return to baseline values were detected in 1 dog with contrast filling of the pancreatic parenchyma.

Conclusions and Clinical Relevance—Results indicated that ERP appears to be a safe imaging technique of pancreatic ducts in healthy dogs, although it induced a transient increase in serum values of pancreatic enzymes. In dogs, repeated clinical examinations and serum enzyme determinations can be used to monitor ERP-induced complications such as acute pancreatitis. ( Am J Vet Res 2004;65:616–619)

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in American Journal of Veterinary Research

canine trypsin-like immunoreactivity concentration was measured in all patient samples at the time of enrollment to evaluate dogs for EPI, which is another cause of cobalamin deficiency Dogs with a serum concentration for canine trypsin-like

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in American Journal of Veterinary Research

peptide to creatinine has also been evaluated; however, none of these assays is sensitive in the detection of pancreatitis. 4,5 Canine trypsin-like immunoreactivity concentration has also been used to detect pancreatitis, but the sensitivity of this test

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in American Journal of Veterinary Research

= Canine trypsin-like immunoreactivity. NA = Not applicable. RR = Reference range. Table 2— Results of hematologic analysis for 67 dogs with gastric histopathologic abnormalities included in the retrospective case series

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in Journal of the American Veterinary Medical Association

. Waritani T , Okuno Y , Ashida Y , Development of a canine trypsin-like immunoreactivity assay system using monoclonal antibodies . Vet Immunol Immunopathol . 2002 ; 87 ( 1-2 ): 41 - 49 . doi: 10.1016/s0165-2427(02)00120-4 49. Viviano KR

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