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measurement of canine pancreatic lipase immunoreactivity in serum . Can J Vet Res 2003 ; 67 : 175 – 182 . 9. Steiner JM Rutz GM Williams DA . Serum lipase activities and pancreatic lipase immunoreactivity concentrations in dogs with exocrine

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in American Journal of Veterinary Research

Abstract

Objective—To develop and validate a radioimmunoassay (RIA) for measuring canine pancreatic lipase immunoreactivity (cPLI) in serum obtained from dogs.

Sample Population—Serum samples from 47 healthy dogs.

Procedures—Canine pancreatic lipase (cPL) was purified from pancreatic specimens of dogs. Antibodies against cPL were raised in rabbits and purified by use of affinity chromatography. A tracer was produced by iodination of cPL with 125I. An RIA was established and validated by determination of sensitivity, working range, dilutional parallelism, spiking recovery, and intra- and interassay variability. A reference range for cPLI in serum was established by use of the central 95th percentile for samples obtained from 47 healthy dogs.

Results—Sensitivity and upper limit of the working range were 0.88 and 863 µg/L, respectively. Observed-to-expected ratios for serial dilutions ranged from 84.9 to 116.5% for 4 samples. Observedto- expected ratios for spiking recovery ranged from 82.8 to 128.6% for 4 samples. Coefficients of variation for intra-assay variability for 4 serum samples were 18.3, 4.2, 3.5, and 8.9%, whereas interassay coefficients of variation were 29.2, 6.2, 3.9, and 4.4%, respectively. The reference range was 4.4 to 276.1 µg/L.

Conclusions and Clinical Relevance—We conclude that the RIA described is sensitive, linear, accurate, precise, and reproducible, with limited accuracy in the high end of the working range and limited precision and reproducibility in the low end of the working range. Additional studies are needed to evaluate whether this degree of accuracy, precision, and reproducibility will negatively impact clinical use of this assay. (Am J Vet Res 2003;64:1237–1241)

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in American Journal of Veterinary Research

validated as reference ranges for this species. cPLI = Canine pancreatic lipase immunoreactivity. cTLI = Canine trypsin-like immunoreactivity. Figure 1 Box and whisker plot of (A) cobalamin, (B) folate, (C) canine pancreatic lipase

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in American Journal of Veterinary Research

. ABBREVIATIONS α 1 -PI α 1 -Proteinase inhibitor CCECAI Canine chronic enteropathy clinical activity index CIBDAI Canine inflammatory bowel disease activity index cPLI Canine pancreatic lipase immunoreactivity CRP C-reactive protein FRD Food

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in Journal of the American Veterinary Medical Association

(both acute and chronic) in dogs. Abbreviations CCK Cholecystokinin cPLI Canine pancreatic lipase immunoreactivity cTLI Canine trypsin–like immunoreactivity CV Coefficient of variation MCT Medium-chain triglyceride a. Steiner

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in American Journal of Veterinary Research

declare that there were no conflicts of interest. Abbreviations cPLI Canine pancreatic lipase immunoreactivity cTLI Canine trypsin-like immunoreactivity EPI Exocrine pancreatic insufficiency RER Resting energy requirement RI Reference

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in American Journal of Veterinary Research

-responsive diarrhea. Serum C-reactive protein concentration was high in 13 of 18 dogs with PLE and 2 of 18 dogs with FRD. Serum concentration of canine pancreatic lipase immunoreactivity was high in 3 dogs with PLE but not in any dogs with FRD. Serum α 1 -proteinase

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in Journal of the American Veterinary Medical Association

pancreatic lipase immunoreactivity measurement was performed and interpreted as previously described, if clinically indicated. 9,10 Canine pancreatic lipase immunoreactivity was measured if dogs had vomiting, diarrhea, anorexia, or signs of abdominal pain

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in Journal of the American Veterinary Medical Association

, 8.5 to 28.5 mg/dL) and chloride (105 mmol/L; reference range, 108 to 126 mmol/L), and high total CO 2 concentration (28 mmol/L; reference range, 14 to 24 mmol/L). A canine pancreatic lipase immunoreactivity test was performed and serum pancreatic

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in Journal of the American Veterinary Medical Association

genetic variants are warranted. ABBREVIATIONS CI Confidence interval cPL Canine pancreas-specific lipase cPLI Canine pancreatic lipase immunoreactivity OR Odds ratio SPINK1 Serine protease inhibitor, Kazal type 1 a. Veterinary

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in American Journal of Veterinary Research