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if Ct values were below 35. qPCR for bacteria Total DNA was extracted from nasal secretion samples using the MagMAX-96 Viral RNA Isolation Kit AM1836 (ThermoFisher Scientific) and purified using Program AM1836-DW-One. Each sample was tested

Open access
in American Journal of Veterinary Research

T he ocular surface is a nutrient-rich environment hosting a wide range of microorganisms. The conjunctival sac microbiome is predominantly composed of commensal bacteria that contribute to the preservation of normal eye health by performing

Open access
in American Journal of Veterinary Research

also important so that the resident gastrointestinal microbiota of reptiles, which aid in optimal digestion and immune function, are maintained or minimally disrupted. 5 Confirmation of a bacterial infection requires the isolation of bacteria from a

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in Journal of the American Veterinary Medical Association

subset of cats with S-CCHS or obstructive cholangiopathies have bacterial infection. 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 However, detection of bacteria by culture may be compromised by the common

Open access
in Journal of the American Veterinary Medical Association

As a result of their selective antibacterial properties, antimicrobials provide selection pressure that favors bacteria that survive therapeutic concentrations of specific drugs. This survival-of-the-resistant phenomenon contributes to the

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in American Journal of Veterinary Research

and disinfection of footwear on limiting mechanical transmission of bacteria on floors in veterinary hospitals and animal facilities has been studied with favorable results reported. 5,12–14,17,18 The University of Wisconsin VMTH houses both the LAH

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in Journal of the American Veterinary Medical Association

immunosuppressive medications lessen or eliminate recurrent episodes of uveitis in affected horses. 13,14 Therefore, the role of bacteria in the pathogenesis of ERU in horses remains unknown. Results of a recent study 15 on herpes simplex virus, varicella zoster

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in American Journal of Veterinary Research

Apopulation of bacteria and fungi normally exist on the canine ocular surface. Gram-positive aerobes are most common, with Staphylococcus spp, Bacillus spp, Corynebacterium spp, and Streptococcus spp predominating. 1–7 Gram-negative and

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in American Journal of Veterinary Research

in people, 15–17 but data for veterinary patients are limited. 18–21 Results of studies 9,10 have shown reduced adherence of bacteria clinically relevant to human patients on triclosan-coated sutures in vitro, but to the authors’ knowledge, the

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in American Journal of Veterinary Research

S everal studies 1 – 4 have demonstrated the increased prevalence of resistance to critical antimicrobial drugs among bacterial isolates from canine veterinary patients. Of particular importance are bacteria that produce extended spectrum beta

Open access
in American Journal of Veterinary Research