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Currently, measuring SAA concentrations used in many studies 11 , 12 is performed using an automated turbidimetric based on a mixture of anti-human SAA-specific monoclonal and polyclonal antibodies assays (LZ-SAA). Recently, the demand for improved
(Appendix). 14,15,17,21,22 Products amplified by a PCR assay were analyzed by gel electrophoresis by use of a 2% (wt:vol) agarose gel in 1X triphosphate EDTA buffer at 100 V. Gels were stained with ethidium bromide, developed with ultraviolet irradiation
-14 disorders. In the past, clinical use of CRP concentration assessments in dogs has been stifled by the lack of an automated assay. For many years, the only commercially available assay was an ELISA. a Unfortunately, that canine CRP ELISA is associated with
. 5 The number and breadth of available tests and methodologies can make it challenging to select and understand the appropriate use of each assay in clinical practice. The purpose of this article is to review the various pancreatic lipase assays that
,2,4,6 Standard coagulation tests such as PT and APTT quantify time to clot formation, but lack sensitivity for decreased coagulation times in hypercoagulable states. 7 Quantification assays of individual components of the coagulation system such as antithrombin
laboratory expertise for accurate identification. 3,4 It is common in human clinical laboratories to diagnose mycological infections via in vitro amplification and detection of fungal DNA via molecular techniques. 5–8 These assays have the advantages of
condition and detection of the carrier state for gangliosidosis in cats have been evaluated by selective assay for enzyme activity. This approach is severely limited with regard to sample availability and consistency because enzyme assay requires material
study 4 indicate that these positive serologic assay results may persist for as long as 6 months. Transmission of BLV in the perinatal period can occur in utero or through the ingestion of infected colostrum. 5–8 Among bovids, in utero infection of
This broad range of disease manifestations over time suggests that a testing algorithm incorporating complementary assays, rather than a single assay focused predominantly on humoral or cellular immunity, will be needed to provide diagnostic accuracy
pathogen shed in feces by use of an L intracellularis –specific PCR assay and analysis of serum samples via IPMA. Both tests are commonly used in swine and are highly specific; sensitivity is high for the IPMAs and variable for the PCR assays. 9
