Search Results
TM GI Parasite PCR) 14 was used to detect Ancylostoma spp dog samples, with concurrent BZ resistance markers, and obtain frequency data. This research describes a newly recognized change in local resistance patterns (Canada) and highlights global
on the observer, and are highly accurate for detecting D immitis . Diagnostic methods using DNA amplification, such as quantitative PCR or loop-mediated isothermal amplification (LAMP) PCR, have, therefore, been developed. 8 , 9 LAMP PCR was
microscopy, acid-fast stain (AFS), direct fluorescent antibody (DFA), immunoassays, and PCR assays. 2 When reviewing the published literature, it is difficult to determine which diagnostic test has the greatest sensitivity and specificity as there has not
reliable, highly sensitive, and specific assay for the detection of viable organisms. 2 Molecular techniques such as quantitative PCR (qPCR) have high sensitivity because the process amplifies a single target sequence of DNA. However, this beneficial
to identify Cfv in preputial samples. Current diagnostic techniques available for the detection of Cfv in preputial samples include bacteriologic culture, 8 DFAT, 9 ELISA, 6 and PCR assay. 10,11 Bacteriologic culture with subsequent phenotypic
and may be found in the prepuce, especially in younger bulls. 5 Various PCR assays, including gel and real-time PCR assays, have been developed for identification of T foetus and are used for both initial parasite detection and for confirmatory
the basis of clinical signs and the presence of inclusions (described as Ehrlichia sp inclusions) in granulocytes on Giemsa-stained slides of blood smears. 1,3 Other research has described the use of a PCR assay–based test to detect A
assessment and fecal parasite screening. The copro-PCR (KeyScreen GI Parasite PCR; Antech Diagnostics Inc) 1 detected DNA of Echinococcus multilocularis and Eimeria spp. The latter was considered to indicate coprophagia, as dogs are not definitive hosts
endosymbionts, the exact role of the Wolbachia organisms in the pathological changes associated with heartworm infection is unknown. 8,9 In the study reported here, a broad-range PCR–ESI-MS assay that was designed to detect a wide range of vector
pathogen shed in feces by use of an L intracellularis –specific PCR assay and analysis of serum samples via IPMA. Both tests are commonly used in swine and are highly specific; sensitivity is high for the IPMAs and variable for the PCR assays. 9
