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ELISA, eggs were collected daily. Anti-ERVA and -HRVA IgY were prepared by ammonium sulfate precipitation and lyophilization, and used for in vitro and in vivo analysis, respectively. Purification and lyophilization of egg yolk IgY To precipitate

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in American Journal of Veterinary Research

Depending on the species, the intensity of the cell-mediated response of reptiles follows a seasonal rhythm. 17–19 The humoral response is dominated by immunoglobulins. Antibody types IgM and IgY are described as functionally important, with IgM being the

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in American Journal of Veterinary Research

incubated for 1 hour at 37 °C, after which the plates were washed. A secondary horseradish peroxidase-conjugated goat anti-chicken IgY antibody (anti-chicken immunoglobulin Y [IgY] antibody; Sigma-Aldrich Laboratories) was added (50 μL of a 1:30,000 dilution

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in Journal of the American Veterinary Medical Association

the environment. Coating a dry cat food with an egg product containing anti-Fel d 1 IgY antibodies neutralizes active Fel d 1 in the cat's saliva. Once the antibodies bind to active Fel d 1, it is neutralized and no longer recognized as an allergen

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in Journal of the American Veterinary Medical Association

-associated lymphoid tissue and GALT. 1 Development of the avian immune system begins early during embryogenesis. A growing embryo has passive immunity that is provided by IgY that is transferred from the dam's blood to the egg yolk. 2 Between days 8 and 14 of

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in American Journal of Veterinary Research

system of birds begins early during embryogenesis. Growing embryos are supplied only with IgY, which is transferred from the dam's blood to the egg yolk and accounts for passive immunity. 25 Between days 8 and 14 of embryonic development, prebursal stem

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in American Journal of Veterinary Research

combination of egg Yolk IgY and phosvitin inhibits the growth of enterotoxigenic Escherichia coli K88 and K99 . Curr Pharm Biotechnol . 2017 ; 18 ( 5 ): 400 – 409 . doi: 10.2174/1389201018666170425120036 28443510 11. Fleckenstein JM . Providing

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in American Journal of Veterinary Research