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stirred bioreactors, which can be scaled up (> 100 L) for manufacturing purposes. 9 The use of microcarrier-based 3-D culture has been demonstrated to improve MSC expansion efficiency in equine MSCs. 10 , 11 In addition to improving MSC culture

Open access
in Journal of the American Veterinary Medical Association

Abstract

Objective—To determine the effects of interleukin (IL)-1β on matrix synthesis and degradation by chondrocytes cultured in a 3-dimensional (3-D) gel medium.

Sample Population—Chondrocytes from 7 dogs.

Procedure—Articular chondrocytes were harvested and cultured in 3-D gel medium alone or with 10 or 20 ng IL-1βml that was added beginning on day 0, 3, 6, or 9. On days 3, 6, 12, and 20 of 3-D culture, samples of the liquid medium were evaluated for glycosaminoglycan (GAG), prostaglandin E2 (PGE2), and matrix metalloprotease (MMP)-3 content. The 3-D plug in each well was evaluated for histologic characteristics of viability, cell morphology, and proteoglycan staining, immunohistochemically stained for collagen type II, and spectrophotometrically analyzed for GAG content.

Results—Significant differences for all variables were detected between controls and each IL-1β group, among groups with different IL-1β concentrations, and among groups with IL-1β added at various time points. Chondrocytes exposed to IL-1β had loss of GAG, increased PGE2 and MMP-3 concentrations, and lack of collagen type-II synthesis. These IL-1β effects appeared to be time and concentration dependent.

Conclusions—Addition of IL-1β to chondrocytes in 3- D gel medium results in time- and concentrationdependent effects on matrix synthesis and degradation and provides an appropriate in vitro model for many of the pathophysiologic events associated with osteoarthritis. (Am J Vet Res 2000;61:766–770)

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in American Journal of Veterinary Research

chemosensitivity to carboplatin. We hypothesized that IC 50 values of carboplatin against FISAS and FOSCC cells measured at 72 hours in 3-D culture would fall within the range of carboplatin concentrations known to elute from C-ICSH beads over the same time period

Open access
in American Journal of Veterinary Research
Author:

authors describe EV production using a 3-D culture of equine bone marrow–derived MSCs . The study found that 3-D culture of MSCs on microcarrier beads did not improve overall MSC expansion but did yield more EVs produced per cell. The authors provide

Full access
in Journal of the American Veterinary Medical Association

, mature tissues customized for individual patients. Differentiation medium is distinct among species, tissue structures, and 2-D and 3-D culture mechanisms. 16 , 17 Results of previous studies 18 , 19 , 20 have established a framework for base medium

Free access
in American Journal of Veterinary Research

in vitro results to in vivo environments. 45 In contrast to monolayer cultures, 3-D cultures better develop in vivo characteristics including cellular morphologies, structural organization, intercellular interactions, and gene expression patterns. 46

Open access
in American Journal of Veterinary Research

,21 In the present study, results of the 2-D and 3-D culture systems confirmed that successful differentiation of pASCs and pSSCs into chondrocytes was characterized by expression of typical chondrogenic genes such as Col II and aggrecan, and results of

Full access
in American Journal of Veterinary Research

been reported for other studies on canine chondrocytes in 2-D or 3-D culture, even when higher IL-1β concentrations 6,7 or a combination of several cytokines 4,5 were used. Therefore, we concluded that the addition of 10 ng of IL-1β/mL for 24 hours

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in American Journal of Veterinary Research

of BMDMSCs in pellets or 3-D cultures for a longer period may have resulted in detectable chondral differentiation. Despite the lack of evidence for an effect on chondrogenesis in BMDMSCs in monolayer cultures, treatment with RA at concentrations ≥ 1

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in American Journal of Veterinary Research

of fibrin hydrogels, treatment of meniscal lacerations with MSCs and fibrin glue, 3-D culture conditions and chondrogenesis, and creation of a decellularized tendon as scaffold for regenerative treatment. 48–51 Promoting rapid vascularization is one

Full access
in American Journal of Veterinary Research