Objective—To determine the nucleotide and amino
acid sequence of atrial natriuretic peptide (ANP) in
cats and its typical regions of cardiac expression.
Animals—5 healthy adult mixed-breed cats.
Procedure—Total RNA was extracted from samples
obtained from the left and right atrium, left and right
ventricle, and interventricular septum of each cat. The
RNA was used to produce cDNA for sequencing and
northern blot analysis. Genomic DNA was extracted
from feline blood samples. Polymerase chain reaction
primers designed from consensus sequences of
other species were used to clone and sequence the
feline ANP gene.
Results—The feline ANP gene consists of 1,072
nucleotides. It consists of 3 exons (123, 327, and 12
nucleotides) separated by 2 introns (101 and 509
nucleotides). It has several typical features of eukaryotic
genes and a putative steroid-response element
located within the second intron. Preprohormone
ANP consists of 153 amino acids. The amino acid
sequence of the active form of feline ANP (ANP-30) is
identical to that of equine, bovine, and ovine ANP-30
and differs from that of human, canine, and porcine
ANP-28 only by 2 carboxy-terminal arginine residues.
The ANP mRNA was detected only in the left and
Conclusions and Clinical Relevance—The genetic
and protein structure and principal regions of cardiac
expression of feline ANP are similar to those of other
species. Results of this study should be helpful in
future studies on the natriuretic response in cats to
diseases that affect cardiovascular function.
(Am J Vet Res 2002;63:236–240)