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  • Author or Editor: William H. Ayars Jr. x
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To determine reference ranges for hematologic and serum biochemical variables of bulls residing at an artificial insemination center.


225 healthy Holstein bulls categorized by age into yearling, intermediate age, and adult groups.


Hematologic and serum biochemical analyses were performed on 1 blood and 1 serum sample from each bull.


Significant differences associated with age were identified for 25 of 33 variables. Serum creatinine concentration for clinically normal adult bulls (2.44 ± 0.33 mg/dl) was higher than previously reported reference values for adult cattle. There was a reversal of the segmented neutrophil-to-lymphocyte ratio between yearling (0.85:1) and adult (2.6:1) bulls. This was associated with a significant and marked decrease in absolute numbers of lymphocytes per microliter between yearling (5,801 ± 1,683) and adult (1,307 ± 509) bulls.

Clinical Relevance

Reference values for selected clinicopathologic variables were generated from the data. (Am J Vet Res 1998;59:1386–1391)

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in American Journal of Veterinary Research