Objective—To examine clinical features, laboratory
test results, treatment, and outcome of dogs with
pure red cell aplasia (PRCA) and idiopathic nonregenerative
immune-mediated anemia (NRIMA).
Animals—43 dogs with severe nonregenerative anemia.
Procedure—Medical records of dogs determined to
have PRCA, NRIMA, or ineffective erythropoiesis on
the basis of bone marrow analysis between 1988 and
1999 were reviewed. Criteria for inclusion were ≥ 5-
day history of severe nonregenerative anemia (Hct <
20%; < 60.0 X 103 reticulocytes/µl) with no underlying
diseases. Information was retrieved on signalment,
clinical signs, laboratory test results, treatment, and
Results—Median age of the dogs was 6.5 years.
Spayed females and Labrador Retrievers were significantly
overrepresented. Median Hct was 11% with no
evidence of regeneration (median, 1.5 X 103 reticulocytes/
µl). Direct Coombs' test results were positive in
57% of dogs. Biochemical abnormalities included
hyperferremia and high percentage saturation of
transferrin. Bone marrow findings ranged from PRCA
(5%) to erythroid hyperplasia (55%). Myelofibrosis
was common. Dogs were treated with immunosuppressive
drugs and the response was complete, partial,
and poor in 55, 18, and 27% of the dogs, respectively.
Mortality rate was 28%.
Conclusion and Clinical Relevance—An immunemediated
pathogenesis should be considered in dogs
with severe, nonregenerative anemia, normal WBC
and platelet counts, hyperferremia, mild clinical signs,
and no evidence of underlying disease. Bone marrow
findings range from the rare PRCA to erythroid hyperplasia.
Myelofibrosis is often detected in affected
dogs and may prevent bone marrow aspiration. (J Am
Vet Med Assoc 2000;216:1429–1436)
Objective—To determine accuracy and precision of a
point-of-care hemoglobinometer for measuring hemoglobin
concentration and estimating PCV in horses.
Procedure—Blood samples were obtained from 43
horses examined at a veterinary teaching hospital.
Hemoglobin concentration was measured with the
hemoglobinometer and by means of the standard
cyanmethemoglobin method; PCV was measured by
centrifugation. Blood samples were also obtained
from 12 healthy horses, and PCV of aliquots of these
samples was altered to approximately 5 to 80% by
removing or adding plasma. Hemoglobin concentration
and PCV were then measured.
Results—For samples from the clinic patients, hemoglobin
concentrations obtained with the hemoglobinometer
were less than concentrations obtained with
the cyanmethemoglobin method; however, there was
a linear relationship between concentrations obtained
with the 2 methods. Breed, sex, body weight, and
duration of sample storage did not significantly affect
the difference between hemoglobin concentrations
obtained with the 2 methods. There was a significant
linear relationship between PCV and hemoglobinometer
hemoglobin concentration (PCV = [2.83 X
hemoglobin concentration] − 0.62). For samples from
the healthy horses, a substantial negative bias was
evident with the hemoglobinometer when hemoglobin
concentration exceeded 16 g/dL.
Conclusions and Clinical Relevance—Results suggest
that this hemoglobinometer is reasonably accurate
and precise when used to measure hemoglobin
concentration in blood samples from horses with a
hemoglobin concentration < 16 g/dL. (J Am Vet Med