Objective—To evaluate the effects of various storage
conditions on one-stage prothrombin time (OSPT),
activated partial thromboplastin time (APTT), and fibrinogen
concentration of canine plasma collected for
Sample Population—Plasma from 9 dogs.
Procedure—Whole blood was collected from dogs by
means of jugular venipuncture and centrifuged at
7,300 × g for 20 minutes at 0 C. A plasma extractor
was then used to generate plasma. Aliquots of plasma
were collected in segments of plastic tubing and
in microcentrifuge tubes, and plasma collection bags,
tubing segments, and microcentrifuge tubes were
immediately frozen at –30 C. Additional tubing segments
and microcentrifuge tubes were stored at 2 C.
After 1 week of storage, all samples were thawed,
and OSPT, APTT, and fibrinogen concentration were
measured. Collection bags and microcentrifuge tubes
were refrozen at –30 C, and values were measured
again 30 days after blood collection.
Results—Values for OSPT, APTT, and fibrinogen concentration
did not vary significantly with storage time,
temperature, or container.
Conclusions and Clinical Relevance—Results suggested
that storage for up to 30 days and at 2 C versus
–30 C did not have any significant effect on hemostatic
parameters of canine plasma obtained for transfusion.
(Am J Vet Res 2001;62:734–735)