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  • Author or Editor: Stephen J. Haslett x
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Abstract

Objective

To develop a noninvasive method to detect disaccharide malabsorption in dogs by measuring hydrogen concentration ([H2]) in exhaled breath before and after experimentally induced disaccharide malabsorption.

Animals

8 healthy mixed-breed dogs.

Procedure

[H2] was measured every 30 minutes for 8 hours after administration of disaccharide solutions (lactose, 0.5 g/kg of body weight; lactose, 1.0 g/kg; sucrose, 2.0 g/kg; maltose, 1.5 g/kg; and lactose [0.5 g/kg] and sucrose [2.0 g/kg]) to determine reference ranges of [H2] for each solution, which were compared with [H2] in dogs with experimentally induced disaccharide malabsorption. To induce disaccharide malabsorption, dogs were given a mild overdose of lactose (1.5 g/kg) or a disaccharidase inhibitor. In the latter experiment, acarbose (10 mg/kg, PO) was given with the combination of lactose (0.5 g/kg) and sucrose (2 g/kg), and with maltose (1.5 g/kg).

Results

Overdosing with lactose resulted in [H2] persistently outside the reference range for lactose in 5 of 8 dogs. Acarbose administration resulted in [H2] persistently outside the reference range in 7 of 8 dogs that received a combination of sucrose and lactose but did not consistently affect [H2] after administration of maltose.

Conclusions

Disaccharide malabsorption resulted in [H2] outside the reference ranges in most of the adult dogs studied, suggesting that the technique may be useful in detecting naturally occurring disaccharidase deficiency. (Am J Vet Res 1999;60:836–840)

Free access
in American Journal of Veterinary Research