Objective—To investigate the possibility that variants
in the acidic or basic keratin genes or in desmoglein 1
may cause the clinical manifestation of familial footpad
hyperkeratosis in Irish Terriers.
Animals—11 dogs belonging to 2 related affected
pedigrees of Irish Terriers.
Procedure—Genomic DNA was extracted from blood
samples obtained from each dog. The DNA markers
linked to the genes keratin 2, keratin 9, and
desmoglein 1 were amplified by use of a polymerase
chain reaction technique, and length of the products
was determined by use of an automatic DNA analyzer.
Results—All tested markers yielded information.
None of the markers (genotype) cosegregated with
the clinical status of the dogs (phenotype) in the 2
Conclusions and Clinical Relevance—Mutations in
the genes encoding keratin 2 and 9 as well as
desmoglein 1 are highly unlikely to be the primary
cause of familial footpad hyperkeratosis in Irish
Terriers. (Am J Vet Res 2003;64:715–720)